摘要目的 探讨丙型肝炎患者抗-HCV水平性与病毒基因型的相关性.方法 采用回顾性研究方法,收集2013至2014年上海市15家医疗机构抗-HCV阳性标本603份,应用雅培Architect I1000免疫分析系统进行复检.对复检阳性标本检测HCV RNA及进行基因分型,并以患者的抗HCV浓度水平的四分位间距为区间,分析不同基因型患者抗HCV浓度水平的分布规律,探讨机体对病毒应答的抗体产生与病毒基因型的相关性.各基因型在不同抗体浓度区间分布率比较采用x2检验.结果 603份标本中抗HCV双试剂复检阳性412份(68.33％),其中174(42.3％)份标本检测出HCV RNA;174份HCV RNA阳性标本中169份得到丙肝基因型结果.以抗体浓度四分位间距为区间,不同基因型患者中不同抗体浓度患者的分布为:1a型(8份)1/8、11/8、4/8、2/8;1b型(112份)25.9％(29/112)、17.0％(19/112)、25.9％(29/112)、31.3％ (35/112);2a型(14份)3/14、4/14、5/14、2/14;3a型(11份)3/11、6/11、2/11、0/11;3b型(16份)4/16、11/16、1/16、0/16;6a型(8份)为2/8、2/8、1/8、3/8.在HCV患者中不同基因型的HCV患者的抗体浓度不同,1b、2a、6a型患者抗体浓度分布均匀,1a型患者的抗体浓度(13.65)相对较高,3b型的患者的抗HCV水平(8.77)相对较低,各基因型在不同抗体浓度区间分布率有差异(x2=35.2,P＜0.05).结论 HCV患者的抗体水平与病毒基因型可能存在相关性.由于部分基因型的例数较少,相关性需要更大标本量的研究证实.

abstractsObjective To investigate the relationship between anti-HCV antibody level and hepatitis C virus genotype in the patients.Methods Total of 603 anti-HCV positive serum samples were collected during 2013 to 2014 by retrospective research method.HCV RNA were detected in anti-HCV positive samples by repeat test and the genotype were detected in HCV RNA positive samples.The distribution of anti-HCV level in different hepatitis C genotype patients was analyzed and the body's response to viral antibodies and viral genotype correlation with anti-HCV concentration interquartile range was explored.Rates among genotype groups were compared using chi-square test.Results Totally 412 of 603 (68.33％) samples were anti-HCV positive by double reagent screening.174(42.3％) samples were detected as HCV RNA positive.The distributions of different anti-HCV level in different genotype patients were 1a(n =8) 1/8,1/8,4/8,2/8;1b(n =112)25.9％ (29/112),17.0％ (19/112),25.9％ (29/112),31.3％ (35/112);2a(n =14)3/14,4/14,5/14,2/14;3a(n =11)3/11,6/11,2/11,0/11;3b(n =16)4/16,11/16,1/16,0/16;6a(n =8)2/8,2/8,1/8,3/8 with anti-HCV concentration interquartile range respectively.The anti-HCV concentration distribution was different in patients with different HCV genotypes.The anti-HCV concentration distribution in patients of 1 b,2a and 6a genotypes were evently,while anti-HCV level was relatively high in 1a (13.65) and relatively low in 3b (8.77).There were differences in different genotypes of antibody concentrations (x2 =35.2,P ＜ 0.05).Conclusions There was correlation between anti-HCV level and HCV genotype.Because there were fewer cases in some genotypes,it was necessary to investigate more samples to corfirm the above results.