摘要目的：探讨血清唾液酸在脑胶质瘤辅助诊断中的价值。方法回顾性研究。收集2014年10月至2015年3月首都医科大学附属北京天坛医院确诊的95例不同病理级别（Ⅰ～Ⅳ级）脑胶质瘤患者（脑胶质瘤组）、175例脑部良性肿瘤患者（脑部良性肿瘤组）及400名健康对照者（健康对照组）血清，在全自动生化分析仪采用酶法检测血清唾液酸含量，采用ANOVA方差分析及SNK-q检验进行各组间比较。绘制ROC曲线，计算最佳诊断临界值及相应的敏感度、特异度、阳性预测值、阴性预测值，以判断血清唾液酸对脑胶质瘤的辅助诊断价值。另外，从门诊和体检科分别收集30例脑胶质瘤和30名健康对照者的血清标本，对计算得到的最佳诊断临界值进行临床验证。结果脑胶质瘤组、脑部良性肿瘤组及健康对照组血清唾液酸含量分别为（0.66±0.14） g／L、（0.61±0.09）g／L、（0.54±0.07）g／L，脑胶质瘤组血清唾液酸含量显著高于脑部良性肿瘤组（q＝6.74，P＜0.05）及健康对照组（q＝16.42，P＜0.05），4个不同病理级别脑胶质瘤血清（Ⅰ级8例，Ⅱ级32例，Ⅲ级24例，Ⅳ级31例）之间差异无统计学意义（F＝1.67，P＞0.05），低级别组（Ⅰ级和Ⅱ级）和高级别组（Ⅲ级和Ⅳ级）间血清唾液酸含量差异无统计学意义（t＝0.55， P＞0.05），但与低级别组相比，高级别组血清唾液酸含量有增高趋势。血清唾液酸诊断脑胶质瘤的ROC曲线下面积为0.79，最佳诊断临界值为0.61 g／L，敏感度为67.74％，特异度为80.69％，阳性预测值为44.68％，阴性预测值为90.76％。以血清唾液酸0.61 g／L为诊断临界值，对30例脑胶质瘤和30例健康对照者进行检测，结果显示敏感度为63.30％，特异度为83.30％。结论血清唾液酸在脑胶质瘤有较好的特异度及阴性预测值，可能是一个有价值的诊断指标。（中华检验医学杂志，2016，39：201-204）

abstractsObjective To discuss the diagnostic value of serum sialic acid for glioma.Methods A retrospective study was conducted.The levels of sialic acid in serum samples of 95 glioma patients, 175 patients with brain benign tumor and 400 normal persons from October 2014 to March 2015 were detected by automatic biochemistry analyzer using enzymic method.The SNK-q test and analysis of variance were used to compare the difference of the groups.By making receiver operating characteristic ( ROC) curve, the cut-off value, sensitivity, specificity, positive predictive value and negative predictive value were calculated to assess the diagnostic value of serum salivary acid.Then the cut-off value was validated by using the serums of 30 glioma patients and 30 normal persons who were out-patients and healthy controls.Results The levels of serum sialic acid in patients with gliomas, patients with brain benign tumor and healthy individuals were (0.66 ±0.14 ) g/L, (0.61 ±0.09 )g/L, (0.54 ±0.07 )g/L.The serum salivary acid of glioma patients were higher than brain benign tumor patients (q=6.74,P<0.05) and normal persons (q=16.42,P<0.05), and there was no significant difference (F=1.67,P>0.05) among the glioma patients of different grades (8 of gradeⅠ,32 of gradeⅡ,24 of grade Ⅲ,31 of grade Ⅳ).There was no significant difference between the low grade patients (gradeⅠandⅡ) and the high grade patients (gradeⅢandⅣ) (t=0.55,&nbsp;P>0.05), but the level of serum sialic acid of high grade group had an increasing trend than the low grade group.The area under the ROC curves was 0.79.The cut-off value of serum salivary acid for diagnosing glioma was 0.61 g/L.The sensitivity was 67.74%, and the specificity was 80.60%.The positive predictive value was 44.68%, and the negative predictive value was 90.76%.Then the cut-off value was used as a diagnostic criteria, and the detected results of 30 glioma patients and 30 normal persons showed that the sensitivity was 63.30% and the specificity was 83.30%.Conclusions The serum sialic acid has good specificity and negative predictive value for diagnosing glioma.It may be a valuable diagnostic marker.