换一批
换一批沙眼衣原体分子生物学检测技术进展及应用
Recent advances of molecular biology technology in Chlamydia trachomatis detection
摘要沙眼衣原体(CT)是临床上引起沙眼和性传播疾病的主要病原体.本文阐述了CT分子生物学检测技术的进展与应用,主要包括荧光定量PCR技术、核酸恒温扩增技术、PCR-杂交技术、测序技术,并比较不同方法的优缺点,同时介绍了CT的即时检验方法.
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abstractsChlamydia trachomatis (CT) is a prevalent pathogen clinically causing trachoma and sexually transmitted diseases. This review presents the progress and application of molecular biology techniques in CT detection according to different types of methodologies, mainly including real-time PCR amplification tests, nucleic acid isothermal amplification tests, PCR-hybridization, sequence analysis and compare their advantages and disadvantages. The recent developments of the point-of-care testing for CT are also briefly introduced in this paper.
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关键词
沙眼衣原体聚合酶链反应核酸扩增技术微阵列分析多位点测序分型Chlamydia trachomatisPolymerase chain reactionNucleic acid amplification techniquesMicroarray analysisMultilocus sequence typing
主题词
沙眼衣原体(Chlamydia trachomatis)分子生物学(Molecular Biology)聚合酶链反应(Polymerase Chain Reaction)核酸扩增技术(Nucleic Acid Amplification Techniques)微阵列分析(Microarray Analysis)
栏目名称
综述
DOI
10.3760/cma.j.issn.1009-9158.2019.12.017
发布时间
2020-01-10
基金项目
深圳市科技计划项目(JCYJ201404160855446)
Shenzhen Science and Technology Program
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