摘要侧流免疫层析技术是一种基于层析膜和纳米标记物的即时检测技术,因其简单、快速和低成本被广泛应用于生物医学、病原体检测、食品安全等多个领域。传统的侧流免疫层析依赖于肉眼观察,灵敏度较低,只能提供定性或半定量结果。随着不同类型标记物和灵敏检测设备的应用,侧流免疫层析实现了对待测物的定量、多组分检测。本文旨在结合纳米标记物、层析膜、条带检测仪,阐述侧流免疫层析检测系统的最新研究进展,以期为标记物的选择,层析膜和检测仪器的改进提供依据。
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abstractsLateral flow immunochromatography is a real-time detection technology based on chromatographic membranes and nano-markers. Because of its simplicity, fastness and low cost, it is widely used in many fields, such as biomedicine, pathogen detection and food safety. Traditional lateral-flow immunochromatography relies on the naked eye, that has lower sensitivity, and can only provide qualitative or semi-quantitative results. With the application of different types of markers and sensitive detection equipment, lateral flow immunochromatography has enabled the quantitative and multi-component detection of analytes. The purpose of this paper is to describe the latest research progress of lateral flow immunochromatographic detection systems in combination with nano-labels, chromatographic membranes and band detectors, with a view to providing a basis for the selection of markers, improvement of chromatography membrane materials and detection instruments.
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