摘要目的 探讨人脂肪间充质干细胞在可降解骨基质材料聚乳酸-羟基乙酸(PLGA)上的黏附、增殖和分化能力,为进一步体内回植提供实验依据.方法 等比混合聚乳酸(PLA)和聚乙醇酸(PGA),有机溶剂注模法制备PLGA.体外培养人脂肪间充质干细胞,复合PLGA,通过扫描电镜观察人脂肪间充质干细胞在PLGA上的黏附、增殖,以及在成脂诱导情况下细胞形态的变化.结果 合成的PLGA呈多孔状,孔隙率为85 % ,孔径为100～400 μm.人脂肪间充质干细胞可在PLGA上黏附、增殖、分泌细胞外基质,并可在成脂诱导剂的作用下分化为圆形的成脂样细胞.复合后14 d,人脂肪间充质干细胞和细胞外基质已将基质材料颗粒完全覆盖.结论 人脂肪间充质干细胞能在PLGA上黏附、增殖,PLGA可作为人脂肪间充质干细胞的载体和组织工程的支架材料.

abstractsObjective To study the ability of adhesion, proliferation and differentiation of human adipose mesenchymal stern cells,which were cultured in PLGA,and thus to provide a basis for their in vivo implantation.Methods PLA was mixed with PGA in a ratio of 1∶ 1,and PLGA was obtained by insert moldling with organic solvents.In vitro, human adipose mesenchymal stem cells were adhered to PLGA.The attachment and the proliferation of human adipose mesenchymal stem cells in PLGA were analysed by scanning electron microscope,and the morphological change of the cells was investigated before and after induction.Results Human adipose mesenchymal stem cells were able to attach, proliferate and secrete extracellular matrix in porous PLGA (pore size:100～400 pm) with porosity of 85 %, and to differentiate into round lipoblast-like cells under the induction of adipose medium.After 14 days, the particulate of PLGA was overlaid by extracellular matrix .Conclusion Human adipose mesenchymal stem cells have the capacity to adhere to PLGA and proliferate, indicating that PLGA can be used as a carrier for human adipose mesenchymal stem cells in adipose tissue engineering.