摘要目的 通过观察强脉冲光对大鼠皮肤碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)表达的影响,探讨光子嫩肤的分子生物学机制.方法 选择15只远交群(SD)大鼠,每只选3个部位用强脉冲光照射,采用同一照射参数,能量密度34 J/cm2,分为3个脉冲,脉宽各为4、5、6 ms,脉冲延时为20 ms及25 ms.照射后第1、3、5、7、15、30天分别于治疗及非治疗部位切取皮肤样本,免疫组化染色(SABC法)观察.结果 照射后第1天,bFGF未见表达,第3天时出现弱阳性颗粒,随时间增加,bFGF的表达也逐渐增强,第15天时达到高峰并持续至第30天,非治疗部位染色阴性.结论 强脉冲光照射皮肤后可引起bFGF表达的增加,提示bFGF在光子嫩肤过程中对皮肤的重塑起重要作用.

abstractsObjective To observe the effect of intense pulsed light (IPL) on basic fibroblastic growth factor (bFGF) expression in rat skin and to elucidate the biological mechanisms of photorejuvenation.Methods The three regions were selected and treated with IPL in 15 SD rats.Treatment parameters were the same:34 J/cm2 of the fluence,triple pulses (4 ms,5 ms,and 6 ms duration),20 ms and 25 ms of pulse delays.At days 1,3,5,7,15,and 30 after treatment,skin specimens were removed at treated areas and non-treated areas,respectively.The expression of bFGF was investigated by means of immunohistochemistry.Results At treated regions,positive stains were not observed in skin cells at day 1.Weaker stains in skin cells were observed at day 3.With time,stains got gradually stronger and reached highest at day 15 and maintained this situation to day 30.At non-treated areas,cells stains were negative.Conclusion IPL technology can enhance the expression of skin bFGF,suggesting that bFGF play a role in remodeling of skin in photorejuvenation.