摘要目的 探讨血小板源生长因子(platelet derived growth factor,PDGF)对瘢痕成纤维细胞生物学行为的影响,及其对瘢痕形成和增生的作用机制,为临床预防和治疗瘢痕提供分子及病理学水平的理论依据.方法 对瘢痕成纤维细胞及正常皮肤成纤维细胞进行体外培养,采用倒置显微镜、四甲基偶氮唑盐(MTT)测定、流式细胞术等方法进行观察、分析,以研究细胞因子PDGF对瘢痕成纤维细胞生物学行为的影响.结果 测定结果显示,与相应的对照组相比,加入细胞因子PDGF后,均显示对细胞起明显的促增殖作用.结论 细胞因子PDGF对成纤维细胞的生长趋势与增殖活性有明显的促进作用,刺激了胶原纤维的合成、增生和沉积.

abstractsObjective To explore the mechanism of cytokines for the scars,and to study the effect of platelet derived growth factor(PDGF)on the biological behavior of fibroblasts in scars.Methods Fibroblasts of scars and normal skins were cultured in vitro.The results were observed and analyzed by light inverted microscopy(LM),and 3-(4,5-dimethyl-thiazol-2-yl)-2,5 ciphenyl tetrazolium bromide (MTT)assay.The effects of PDGF on the biological behaviors of fibroblasts of scars were also determined. Results In vitro study,using LM,FCM and MTT assay,showed that proliferation of fibroblasts were inereased significantly when PDGF was added to the cultures,as compared to the control groups.Conclusions PDGF can increase fibroblast proliferation.These results demonstrate that PDGF is beneficial for wound healing at early stage.