摘要目的 探讨锌指基因217(ZNF217)和翻译延伸因子1α(EF1α)在病理性癜痕中的表达及其相互关系,以及它们在瘢痕形成中的作用及机制,为瘢痕防治提供实验依据.方法 利用实时荧光定量聚合酶链式反应(PCR)法、蛋白质免疫印迹(Western印迹)法检测正常皮肤、成熟瘢痕、增生性瘢痕和瘢痕疙瘩组织中ZNF217和EF1α的mRNA、蛋白相对表达水平.结果 正常皮肤、成熟瘢痕、增生性瘢痕和瘢痕疙瘩组织中ZNF217的mRNA及蛋白质相对表达量分别为1.46±0.397、1.45±0.265、4.49±0.999、5.47±0.808;0.276±0.0211、0.299±0.0150、0.743±0.0509、0.747±0.0377.EF1α的mRNA及蛋白质相对表达量分别为1.47±0.469、1.47±0.218、5.10±1.680、5.74±1.920;0.505±0.0371、0.518±0.0153、0.780土0.0369、0.792±0.0290.病理性瘢痕组织中ZNF217的mRNA及蛋白质表达显著高于正常皮肤、成熟瘢痕(P＜0.01)；病理性瘢痕组织中EF1α的mRNA及蛋白质表达增高,与正常皮肤、成熟瘢痕对照组比较,差异有统计学意义(P＜0.01)；病理性瘢痕组织中ZNF217的mRNA及蛋白质表达与EF1α的mRNA及蛋白质表达呈正相关.结论 ZNF217在病理性瘢痕组织中表达增高,可能通过调节EF1α等细胞周期调控因子而促进瘢痕组织中细胞的增生,对病理性瘢痕的形成可能起着重要作用.

abstractsObjective To study the expression of ZNF217 and EF1α gene in the pathological scars and to investigate role and probable mechanism in the pathogenesis of abnormal scar.Methods Quantitative real-time PCR and Western blot were performed to detect the expression and distribution of mRNA and protein of ZNF217 and EF1α in hypertrophic scar (10 cases),keloid (10 cases),normal scar (10 cases),and normal skin (10 cases),and statistics was used to analyze the data.Results The expression of ZNF217 mRNA and protein in the normal skin,normal scar,hypertrophic scar and keloid were 1.46±0.397,1.45±0.265,4.49±0.999,5.47±0.808; 0.276±0.0211,0.299±0.0150,0.743t0.0509 and 0.747±0.0377,respectively.The expression of EF1α mRNA and prorein in the normal skin,normal scar,hypertrophic scar,and keloid were 1.47±0.469,1.47±0.218,5.10±1.68,5.74±1.92; 0.505±0.0371,0.518±0.0153,0.780±0.0369 and 0.792±0.0290,respectively.The positive rate of mRNA and protein of ZNF217 and EF1α was not statistically different between the hypertrophic scar and keloid (P＞0.05),while they were all remarkably significant in comparison between normal scar and abnormal scar (P＜0.01).In pathological scar mRNA and protein of ZNF217 and EF1α showed a strong positive correlation.Conclusions The expression of ZNF217 and EF1α is increased in pathological scar.Therefore,ZNF217 and EF1α overexpression may play an important role in the proliferation of fibroblasts and in the pathogenesis of pathological scar.