摘要目的 探讨湿润烧伤膏应用对超脉冲CO2点阵激光干预兔耳浅表性瘢痕真皮-表皮连接(DEJ)区的组织学及创面愈合的影响.方法 用超脉冲CO2点阵激光干预兔耳浅表性瘢痕,采用传统络合碘治疗和湿润烧伤膏治疗两种方法对创面进行修复.分别于第1周、第2周、第4周、第8周切取组织标本,采用电镜观察两组DEJ区超微结构改变,光镜观察真皮乳头和PAS染色表皮基底膜组织学变化.结果 湿润烧伤膏组在创面愈合时间上短于传统络合碘组(P＜0.05),湿润烧伤膏组第8周半桥粒和锚纤维数量明显多于第1周,络合碘组第8周半桥粒和锚纤维数量明显多于第1周,组间差异有统计学意义(P＜0.05).络合碘组半桥粒、锚纤维等超微结构再生数量比湿润烧伤膏组明显减少,组间差异有统计学意义(P＜0.05).结论 超脉冲CO2点阵激光可引起瘢痕DEJ区超微结构的组织学改变,湿性愈合环境有利于DEJ区组织的修复重建,对瘢痕皮肤的功能重建具有积极的意义.

abstractsObjective To explore the effects of wound healing and histological changes by utilizing ultrapulse CO2 fractional laser for the treatment of superficial scar in dermal-epidermal junction (DEJ) in rabbit ears.Methods We adopted traditional iodophor treatment and moist exposed burn ointment treatment repairing the wound and utilizing ultra pulse CO2 fractional laser for the treatment of rabbit ears superficial scar.The superficial scars in rabbit ear were induced with ultra pulse CO2 fractional laser.In 1,2,4,8 weeks tissue samples would be taken.Using electron microscopy we observed DEJ ultrastructure and light microscopy to observe the histological changes of the dermal papilla and PAS staining for epidermis basement membrane.Results The wound healing time of moist exposed burn ointment group was shorter than that of iodophor group (P＜0.05).The number of hemidesmosomes and anchoring fibers in the moist exposed burn ointment group on the 8th week was more than the 1th week,The number of hemidesmosomes and anchoring fibers in the iodophor group on 8th week was more than 1th week (P ＜0.05).The number of hemidesmosomes and anchoring fibers in the iodophor group was less than the moist exposed burn ointment group (P＜0.05).Conclusions U1trapulse CO2 fractional laser can cause ultra structural changes in scarring dermal-epidermal junction area,wet wound healing environment promotes the reconstruction of DEJ district organization,and rebuilding the function of the scar skin has the positive significance.