摘要目的 探讨富血小板血浆(PRP)对大鼠光老化皮肤的改善作用.方法 通过紫外线照射F344大鼠建立皮肤光老化模型.模型动物分为A-PRP组(注射激活PRP)、N-PRP组(注射未激活PRP)、生理盐水组(注射生理盐水);未干预组(仅照射建模)、正常大鼠组(仅剃除毛发)5个组.干预4周后,对大鼠皮肤大体外观、组织学特征进行评估.结果 A-PRP组、N-PRP组大鼠皱纹、色素沉着等情况较生理盐水组、未干预组明显改善,4个组均未恢复至正常大鼠组水平.组织学观察A-PRP组、N-PRP组大鼠较生理盐水组、未干预组大鼠皮肤胶原增生明显,排列规律,炎症细胞浸润减少,真皮厚度显著增加,差异有统计学意义(P＜0.05).A-PRP组组织学特征略优于N-PRP组,A-PRP组真皮厚度高于N-PRP组,差异有统计学意义(P＜0.05);生理盐水组、未干预组比较,差异无统计学意义(P＞0.05);正常大鼠组真皮厚度显著高于生理盐水组,差异有统计学意义(P＜0.05).结论 注射PRP能够改善大鼠光老化皮肤外观及组织学特征,A-PRP效果优于N-PRP.

abstractsObjective To observe the effect of platelet-rich plasma (PRP) on photoaging skin of rat.Methods F344 rats were used as model animals and skin photoaging model was established by UV irradiation.The model animals were divided into 5 groups:group A was injected with activated PRP (A-PRP);group B was injected with inactive PRP (N-PRP);group C was injected with normal saline;group D was irradiated only with UV;Four weeks after the injection,the appearance and histological characteristics of the rat's skin were evaluated.Results Compared with group C and group D,the wrinkles and skin color in group A and group B were significantly improved.Histological observation showed that the histological features of group A and group B were significantly better than that of group C and D (P＜0.01).The thickness of dermis in group A was higher than that in group B (P＜0.05).There was no significant difference between groups C and D (P＞0.05).The thickness of dermis in group E was significantly higher than that in group C (P＜0.01).Conclusions Injection of PRP can improve the appearance and histological features of photo-aged skin in rats.A-PRP is better than N-PRP.