摘要目的 探讨40 U/ml、100 U/ml两种浓度肉毒毒素在人体皮肤内注射后的弥散范围,为临床应用提供支持.方法 将40 U/ml、100 U/ml高低两种浓度的肉毒毒素溶液注射于受试者真皮内,各注射4个点,每点4 U(40 U/ml为0.1 ml,100 U/ml为0.04 ml),分别于注射后2d、5d、14 d、1～9个月在注射区域做碘淀粉-出汗试验,测量无汗区范围,对两种浓度的注射效果进行比较.结果 注射后2d注射点周围出现圆形无汗区,注射后14 d达到最大、边界最清晰;6个月出现边缘模糊,9个月时完全消失.在上述不同测量时间点,高浓度(100U/ml)药液无汗范围分别为113、345、530、380、330、255、285、295、295 mm2;低浓度(40 U/ml)药液无汗范围分别为113、345、450、345、285、255、255、255、255 mm2,两种浓度无汗范围比较,差异无统计学意义(P＞0.05).结论 肉毒毒素皮内注射相同剂量浓度、容量,对作用范围无影响.

abstractsObjective The recommended concentration of botulinum toxin type A is 40 100 U/ml.This study observed diffusion areas of two different concentration (40 U/ml and 100 U/ml) of botulinum toxin type A with intradermal injection to provide data for clinical application.Methods Intradermal injections with two different concentrations of botulinum toxin type A were conducted in subjects' forearm,with each level 4 points,and 4U per point (40 U/ml as 0.1 ml,100 U/ml as 0.04 ml).Minor's tests were conducted in 2nd day,5th day,2nd week and every month between 1st to 9th month respectively.The anhidrosis areas were measured and then effects of both concentrations were compared.Results The round anhidrosis areas were found in 2nd day,reaching the maximum range nearly at 2nd week,and then gradually dimming from 6th month,and completely vanishing at 9th month.In different measured points,the definite anhidrosis areas of high concentration (100 u/ml) were 113,345,530,380,330,255,285,295 and 295 mm2 respectively,while the low concentration (40 u/ml) were 113,345,450,345,285,255,255,255 and 255 mm2 respectively.The results did not show significant difference.Conclusions Concentration and volume do not show significant influence on areas of sweat gland when botulinum toxin type A is injected intradermally in a certain dose.