摘要目的:探讨真皮内注射二甲氨基乙醇(DMAE)复合制剂(赛雷弗 ?)对D-半乳糖诱导的亚急性衰老大鼠皮肤胶原合成代谢的影响。 方法:2014年4—10月，在青岛大学解剖教研室进行实验研究。30只Wistar雄性大鼠分为衰老对照组、衰老治疗组、正常组，每组各10只。衰老对照组、衰老治疗组两组颈背部皮下注射D-半乳糖125 mg·kg -1·d -1, 42 d。从18 d开始于衰老对照组、衰老治疗组臀背部分别注射生理盐水和赛雷弗 ? 1 ml，每周1次，连续4周。每次治疗后3 d，共聚焦显微镜(RCM)检测大鼠皮肤。42 d取大鼠注射药物区域皮肤，观察各组皮肤真皮厚度、胶原纤维密度、皮肤羟脯氨酸含量变化，RT-PCR法检测皮肤组织Ⅰ、Ⅲ型前胶原(ColⅠ、ColⅢ)、转化生长因子(TGF)β1、Smad3 mRNA表达，以及免疫组织化学检测成纤维细胞增殖细胞核抗原(PCNA)、TGFβ1表达。 结果:RCM显示，随着真皮内注射赛雷弗次数增加，衰老大鼠皮肤胶原纤维的密度逐渐增加。注射4次后，衰老治疗组大鼠真皮厚度、羟脯氨酸含量、TGFβ1 mRNA和蛋白表达高于衰老对照组，但仍低于正常组( F值分别为25.45、98.90、37.94、21.35，均 P<0.05)；胶原纤维密度、ColⅠ、Smad3 mRNA表达高于衰老对照组( F值分别为44.46、29.54、10.01，均 P<0.05)；和正常组比较，差异无统计学意义( P>0.05)。PCNA表达低于正常组，和衰老对照组比较，差异无统计学意义。各组ColⅢ mRNA表达差异均无统计学意义(均 P>0.05)。 结论:真皮内注射赛雷弗可能通过正调节TGFβ1/Smads通路分子表达促进Ⅰ型胶原蛋白合成。

abstractsObjective:To evaluate the effects of cellulift ? administered intradermally by mesotherapy on collagen synthesis in D-galactose induced aging model of rats. Methods:The study was conducted between April and October in 2014 in the Department of Anatomy, Qindao University. 30 male rats were randomly allocated to three groups: aging treatment group, aging control group and normal group; each group had ten rats. Aging treatment group and the control group were subcutaneously injected with D-galactose prepared in saline 125 mg·kg -1·d -1 for 42 day. Normal group was injected with saline for 42 d with same method and dose. From the 18th day after shaving their hair, the dermis of two sides hip skin marked zone of aging treatment group were injected cellulift at a dose of 1 ml per week for 4 weeks. Meanwhile, the aging control group was administrated the same volume of saline with same method. In vivo skin collagen alterations were investigated by reflectance confocal microscopy 3 days after every treatment. Skin specimens were obtained in 42 days. In order to measure the dermal collagen density and dermal thickness, HE and Masson trichrome staining were performed, respectively. Immunohistochemical staining for TGFβ1 and proliferating cell nuclear antigen (PCNA) was performed. Also, the level of TGFβ1, Smad3, types Ⅰ and Ⅲ pro-collagen mRNA expression was assessed by real-time quantitative polymerase chain reaction. Results:As revealed by RCM, collagen density of aging treatment group increased gradually after treatments, while in aging control group it decreased with time. Measurement of dermal thickness, hydroxyproline content and TGFβ-1 mRNA and protein expression in treatment group increased significantly as compared with that in aging control group, but were significantly lower than that in normal group (F values were 25.45, 98.90, 37.94 and 21.35, respectively; P<0.05). Measurement of dermal collagen density, the mRNA expression of type I pre-collagen and Smad3 elevated over that of aging control group with significant difference (F values were 44.46, 29.54 and 10.01, respectively; P<0.05), and there was no difference between normal and aging treatment group ( P>0.05). The difference of PCNA expression between aging control and treatment groups was not significant ( P>0.05), and both were lower than normal group ( P<0.05) . Conclusions:Cellulift ? shows anti-aging effects by activating collagen synthesis and eventually causing dermal thickening. This effect is probably mediated by TGF-β1/Smad3 signaling pathway.