摘要多重连接探针扩增是一种建立在PCR技术七的新型相对定量检测技术,该技术具有高效率、高分辨率、操作简便、设备要求低等诸多优势,目前已被广泛应用于基因片段缺失与重复、点突变及甲基化检测等相关疾病的分子生物学研究.其与基因芯片结合而建立的多重连接探针扩增微阵列芯片技术不但真正具备了高通量的检测能力,还使该技术的可靠性获得了很大的提高.本文就多重连接探针扩增的技术方法 、应用进展及目前还存在的局限性作一综述.

abstractsMuhiplex ligation-dependent probe amplification(MLPA)is a semiquantitatitive analysis based on polymerase chain reaction(PCR).It possesses many advantages such as high efficiency,simple operation,low cost and has been wildly applied in researches of diseases associated with copy number variation,point mutation and methylation.Recently,MLPA is combined with DNA chip to become a real high-throughput method and get great improvement in reliability.Here,the progresses of methods and application of MLPA,as well as its limitations are reviewed.