摘要目的 探讨二氢青蒿素( dihydroartemisinin,DHA)对白血病细胞K562 BCR/ABL融合基因表达的影响及意义.方法 采用噻唑蓝比色方法检测不同浓度的DHA对于K562细胞的增殖抑制作用,并计算不同培养时间点的抑制率.用逆转录PCR检测K562细胞处理前后BCR/ABL融合基因的表达变化,流式细胞仪检测K562细胞的凋亡情况.结果 DHA(10～160 μmol/L)能抑制K562细胞的增殖,随着浓度增加抑制作用明显增强,培养24 h后抑制率从52.76％增加到94.65％;用浓度为20μmol/L的DHA对K562细胞作用12～48 h后,逆转录PCR检测BCR/ABL融合基因表达,△Ct值为4.45±0.25和5.23±0.21.与对照组(4.23±0.21)相比,差异有统计学意义(P＜0.05).结论 DHA可通过影响BCR/ABL融合基因的表达抑制K562细胞的增殖.这可能是导致K562细胞凋亡的原因之一.

abstractsObjective To investigatethe effect of dihydroartemisinin (DHA) on the BCR/ABL fusion gene in leukemia K562 cell.Methods K562 cells were cultured in vitro.The rate of proliferation inhibition of cells treated with various concentrations of DHA were determined by using [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) method.Expression of BCR/ABL fusion gene was analyzed by reverse transcription(RT-PCR) before and after DHA treatment.Apoptosis of K562 cells was detected by flow cytometry.Results The growth of K562 cells was inhibited when the concentrations of DHA were 10～160 μmol/L.With the added dose of DHA,the growth inhibition was remarkable,with the rate of inhibition risen from 52.76％ to 94.65％.The expression of BCR/ABL fusion gene,as detected by RTPCR after incubating the K562 cells with 20μmol/L DHA,measured as△Ct=4.45±0.25 after 12 h and △Ct=5.23±0.21 after 24 h,which was significantly lower compared with that of the control (△Ct=4.23±0.21,P＜0.05).Conclusion DHA can inhibit the proliferation of leukemia K562 cells and facilitate the induction of apoptosis by downregulating the expression of BCR/ABL fusion gene.