摘要目的 提取西安地区出土3000年前人牙齿中的DNA并进行性别鉴定,为后续的考古研究提供帮助.方法 选取西安地区出土的3000年前人牙齿35颗,应用逆向根管技术提取牙齿内部组织的DNA.设计釉原蛋白基因特异性引物,应用PCR技术进行样本的性别鉴定.结果 成功提取到30份3000年前的人基因组DNA,并对相应样本进行了性别鉴定,其中男性8例,女性22例.结论 逆向根管技术可用于古人类牙齿DNA的提取,基因学方法可补充体质人类学方法的不足,完善古代样本性别信息.

abstractsObjective To extracted DNA from ancient human teeth dated 3000 years ago unearthed in Xi'an and determine the genders for the individuals.Methods Thirty-five ancient human teeth were studied.A ‘Reverse-root-canal' technique and a Chelex-100 solution were used to extract the DNA.Specific primers for Amelogenin gene were designed for PCR amplification.Results Genomic DNA was successfully extracted from 30 samples,for which 8 were determined to be males and 22 were females.Conclusion The "Reverse-root-canal" technique can be used for extracting DNA from ancient human teeth.Genetics method can supplement physical anthropology for sex-determination of ancient samples.