摘要目的 观察转录因子T-bet和GATA-3在食管痛患者外周血单个核细胞(PBMC)中的表达,并探讨其与患者免疫状态的关系.方法 采用逆转录-荧光定量-聚合酶链反应(RT-FQ-PCR)检测60例食管癌患者和30例健康对照者PBMC中T-bet和GATA-3的mRNA表达水平,采用酶联免疫吸附法(ELISA法)检测血浆中干扰素γ(IFN-γ)和白细胞介素4(IL-4)的水平.结果 与健康对照组相比,食管癌患者T-bet mRNA表达明显降低(Ⅰ、Ⅱ期食管癌组为0.27±0.05,Ⅲ、Ⅳ期食管癌组为0.12±0.02),GATA-3 mRNA表达明显升高(Ⅰ、Ⅱ期食管癌组为0.45±0.06,Ⅲ、Ⅳ期食管癌组为0.55±0.03),IFN-γ表达明显降低[Ⅰ、Ⅱ期食管癌组为(12.12±1.48)ng/L,Ⅲ、Ⅳ期食管癌组为(8.44±0.90)ng/L],IL-4表达明显升高[Ⅰ、Ⅱ期食管癌组为(18.64±0.77)ng/L,Ⅲ、Ⅳ期食管癌组为(25.28±2.02)ns/L],Ⅰ、Ⅱ期食管癌组与Ⅲ、Ⅳ期食管癌组间差异无统计学意义(均P>0.05).IFN-γ水平与T-bet mRNA表达呈正相关(r=0.79,P<0.05),IL-4与GATA3 mRNA表达呈正相关(r=0.43,P<0.05).结论 食管癌患者外周血中T-bet表达降低,GATA-3表达升高,Th1和Th2平筏被打破,Th2占优势.T-bet和GATA-3参与了Th1和Th2平衡的调控.

abstractsObjective To investigate the expression of transcription factors (IF) T-bet and GATA-3 mRNA in peripheral blood mononuclear cells and its correlation with immune status in esophageal cancer patients. Methods Sixty patients were divided into two groups according to the clinical data: group A consisting of stage Ⅰ and Ⅱ, group B including stage Ⅲ and Ⅳ. The gene expression of T-bet and GATA-3 in 60 esophageal cancer patients and 30 healthy controls was detected by reverse transcription-polymerasc chain reaction (RT-PCR). The expression of IFN-γ and IL-4 was measured by enzyme linked immunosorbent assay (ELISA). Results Expression of T-bet mRNA in esophageal cancer patients(stage Ⅰ and Ⅱ: 0.27±0.05 ng/L, stage Ⅲ and Ⅳ: 0.12±0.02 ng/L)was significantly lower than that in the healthy controls (1.35±0.14 ng/L), but the expression of GATA-3 mRNA in esophageal cancer patients (stage Ⅰ and Ⅱ: 0.45±0.06, stage Ⅲ and Ⅳ: 0. 55±0. 03) was significantly higher than that in the healthy controls (0.09±0.10). The plasma level of Thl cytokine IFN-γin the patients [stage Ⅰ and Ⅱ: (12.12±1.48) ng/L, stage Ⅲ and Ⅳ:(8.44±0.90) ng/L] was significantly lower than that in the healthy controls, while the level of Th2 cytokine IL-4 in the patients [stage Ⅰ and Ⅱ: (18.64±0.77) ng/L, stage Ⅲ and Ⅳ:(25.284±2.02) ng/L] was significantly higher than that in the healthy controls. However, neither in the expression of T-bet and GATA-3, nor in the plasma level of IFN-γ and IL-4, showed a significant difference between group A and B. Conclusion In the peripheral blood of esophageal cancer patients, the expression of T-bet decreased, while GATA-3 increased, Th1/Th2 balance is broken, and the Th2 is dominant. T-bet and GATA-3 play a part role in the regulation of Th1/Th2 balance.