摘要目的 在不同分化的胃癌细胞株中寻找与胃癌分化相关的蛋白质,并对其功能进行初步了解.方法 采用双向电泳及基质辅助激光解吸电离-飞行时间质谱鉴定法,对3种分化程度不同的胃癌细胞株MKN28、SGC7901和BGC823进行了研究,并应用Western blot法对鉴定出的部分蛋白质在胃癌细胞株及胃癌组织中的表达进行验证.结果 在3种分化程度不同的胃癌细胞株MKN28、SGC7901和BGC823中,找到表达差异明显的蛋白质点14个,其中8个蛋白质点得到鉴定,分别为类硫氧还蛋白过氧化物酶、甘油醛-3磷酸脱氰酶、β-微管蛋白多肽、假定蛋白、锌指蛋白139、蛋白酪氨酸激酶、钙网蛋白前体和原肌球蛋白.这些蛋白质与胃癌细胞信号转导、维持细胞稳态、参与糖酵解及肿瘤药物代谢、抗氧化损伤等功能有关.Western blot法检测证实,这些蛋白质在胃癌细胞株及胃癌组织中的表达与蛋白质组学所得结果一致.结论 分化程度不同的胃癌细胞蛋白质表达存在差异,差异蛋白质主要与胃癌细胞的信号转导、维持细胞稳态、参与糖酵解及肿瘤药物代谢、抗氧化损伤等功能有关.

abstractsObjective To investigate the differentiation-related proteins in human gastric carcinoma cell lines by comparative proteomics.Methods The holoproteins of human gastric carcinoma cell lines MKN28,SGC7901 and BGC823 were measured by two-dimensional gel electrophoresis and matrix assisted Laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS).Some proteins identified by proteomics were tested by Western blot in the cell strains and tissues of gastric carcinoma.Results 14 differential protein spots were found in the 3 gastric carcinoma cell lines,among them 8 spots were identified by MALDI-TOF-MS.These proteins were probably thioredoxin peroxidase,glyceraldehyde-3-phosphate dehydrogenase(GAPD),β-tubulin polypeptide,hypothetical protein,zinc finger protein(ZNF)139,protein-tyrosine kinase,calretieulin precursor,and tropomyosin,proteins related with biological behavior of gastric carcinoma cells such as signal transduction,cellular homeostasis,glycolysis,antioxidation action,multidrug resistance(MDR),etc.The expressions of those proteins in gastric cancer cells and tissues identified by Westem blot were consistent with the results obtained by proteomics.Conclusion Differential proteins are found in 3 human gastric carcinoma cell lines,mainly,proteins related with cell signaling,maintenance of homeostasis,glycolysis,metabolism of anti-cancer drug and anti-oxidative injury,etc.