摘要目的 研究碱性成纤维细胞生长因子(bFGF)和第10号染色体缺失性磷酸酶-张力蛋白同源物基因(PTEN)在宫颈癌组织中的表达及临床意义.方法 采用组织芯片和免疫组化技术检测143例宫颈浸润癌(ICC)和20例癌旁正常官颈上皮(NCE)中bFGF和PTEN的表达,分析bFGF和PTEN表达与病理分级、临床分期和淋巴结转移等临床病理因素的关系.采用SPSS 13.0统计软件包进行统计学分析,相关性分析采用Spearman等级相关分析.结果 bFGF在ICC和NCE中的阳性表达率分别为88.8%(127/143)和25.0%(5/20,P=0.000);PTEN在ICC和NCE中的阳性表达率分别为67.1%(96/143)和100.0%(20/20,P=0.000).bFGF的表达与淋巴结转移(r=0.239,P=0.004)和病理分级(r=0.369,P=0.000)呈正相关,PTEN的表达与临床分期(r=-0.189,P=0.024)、病理分级(r=-0.211,P=0.011)和淋巴结转移(r=-0.321,P=0.000)呈负相关.bFGF和PTEN在ICC中的表达强度呈负相关(r=0.261,P=0.002).随着ICC恶性程度增高,PTEN的表达强度逐渐下降,bFGF的表达则呈上升趋势.结论 bFGF和PTEN在ICC中的异常表达与肿瘤的分化、侵袭和发展有关,联合检测bFGF和PTEN可用于评估ICC的生物学行为.

abstractsObjective To investigate the expressions of bFGF and PTEN in cervical carcinoma and their clinical significance. Methods Tissue microarray technique and immunohistochemistry SP method were used to detect the expressions of bFGF and PTEN in 143 cases of invasive carcinoma of cervix ( ICC) and 20 cases of normal cervical epithelium remote from tumor ( NCE ). The relationship between the expressions of bFGF and PTEN in ICC and some factors relating to clinical pathology of cervical carcinoma such as histopathological grading, lymph node metastasis, stroma involvement and FIGO staging were analyzed. Results The rate of the positive expression of bFGF in ICC was significantly higher than that in NCE 88.8% (127/143) vs. 25.0% (5/20, P=0.000). The rate of positive expression of PTEN in ICC was significantly lower than that in NCE 67. 1% (96/143) vs. 100. 0% (20/20, P =0. 000). The expression of bFGF was positively correlated with lymph node metastasis and histopathological grading ( r = 0.239, P = 0. 004 and r = 0. 369, P = 0. 000, respectively). The expression of PTEN was negatively correlated with FIGO staging, histopathological grading and lymph node metastasis (r= -0.189, P= 0.024; r= -0.211,P =0.011; r= -0. 321 ,P =0. 000, respectively). The expression of bFGF was negatively correlated with the expression of PTEN in ICC (r = -0.261 ,P =0.002). Conclusion The overexpression of bFGF and underexpression of PTEN are closely related to the invasion and growth of cervical carcinoma. Detection of the expression of both bFGF and PTEN may be of value in further understanding the biological behavior and predicting the prognosis of cervical carcinoma.