摘要目的 探讨miRNA-106a基因表达水平与食管鳞癌患者临床病理特征和预后的关系.方法 应用实时荧光定量PCR方法检测81例食管鳞癌组织和相应正常组织中miRNA-106a基因的表达量,采用免疫组化法检测p53、人表皮生长因子受体2(HER-2)、DNA拓扑异构酶Ⅱ(TopoⅡ)和多药耐药相关蛋白(MRP)的表达情况,分析miRNA-106a表达与食管鳞癌患者临床病理特征、相关蛋白表达和预后的关系.结果 81例食管鳞癌组织中,miRNA-106a基因低表达48例(59.3％),高表达11例(13.6％),正常表达22例(27.2％).miRNA-106a基因表达水平与食管鳞癌患者的淋巴结转移、病理分期和神经浸润显著相关(均P＜0.05).miRNA-106a基因表达水平与p53蛋白表达显著相关(P =0.006),与HER-2、TopoⅡ和MRP蛋白表达无关(均P＞0.05).miRNA-106a基因表达水平与食管鳞癌患者的总生存时间(0S)无关(P =0.486),与无进展生存时间(PFS)有关(P =0.032).多因素Cox回归分析显示,食管鳞癌患者的PFS与患者年龄、性别、肿瘤长度、T分期、分化程度、神经浸润和miRNA-106a表达水平无关(均P＞0.05),而与淋巴结转移有关(P =0.029).结论 miRNA-106a基因在食管鳞癌组织中低表达,具有抑癌基因的功能,可作为生物标记评估食管鳞癌患者的预后.

abstractsObjective To study the expression of miRNA-106a gene in esophageal squamous cell carcinoma (ESCC) and its association with clinicopathological features and prognosis of ESCC patients.Methods Real-time fluorescence quantitative polymerase chain reaction (PCR) assay was used to determine the expression of miRNA-106a gene in esophageal cancer tissue and corresponding normal mucosa of 81 cases.Immunohistochemical technique was applied to detect the expression of p53,human epidermal growth factor receptor 2 (HER-2),DNA topoisomerase Ⅱ (Topo Ⅱ) and multidrug resistance-associated protein (MRP).The association of miRNA-106a expression with clinicopathological features,expression of related proteins,and prognosis of the patients was analyzed.Results Among the 81 cases,under-expression of miRNA-106a gene was found in 48 cases (59.3％),normal expression in 22 cases (27.2％),and overexpression in 11 cases (13.6％).The expression of miRNA-106 gene was significantly associated with lymph node metastasis,pathological stage,and nerve invasion (all P ＜ 0.05),significantly associated with expression of p53 (P =0.006),and not significantly associated with expressions of HER-2,Topo Ⅱ and MRP proteins (all P ＞ 0.05).The expression of miRNA-106a gene was also significantly associated with progression-free survival (PFS,P =0.032),but not significantly with overall survival (OS,P =0.486).The results of Cox multivariate regression analysis showed that the PFS of ESCC patients was significantly correlated with lymph node metastasis (P =0.029),but not correlated with the age,gender,tumor length,T stage,degree of differentiation,nerve invasion,and miRNA-106a expression (all P ＞ 0.05).Conclusions In esophageal squamous cell carcinomas,the miRNA-106a gene is under-expressed,with tumor suppressor function,and may be regarded as a biological marker to assess the prognosis in patients with esophageal squamous cell carcinoma.