摘要基于实时荧光定量PCR(qPCR)技术的肿瘤分子病理检测具有操作简便、报告周期短、灵敏度高、结果判读标准化等优点。然而，在临床实践中，qPCR技术的性能验证、质量管理、异常结果处理等问题相对复杂，缺乏统一规范和标准。因此，专家共识旨在从qPCR检测全流程角度，对影响检测结果的重要环节、检测过程中遇到的常见问题及异常结果判定及处理等提供规范化意见，达成基于qPCR技术的肿瘤分子病理检测临床实践中国专家共识，以规范检测流程并提高结果准确性，促进技术发展和更为广泛的临床应用。

abstractsQuantitative real-time polymerase chain reaction (qPCR) is one of the most widely used molecular pathological diagnostic techniques in China due to its advantages of the simple operation, short turnaround time, high sensitivity, and standardizable result analysis. However, in clinical practice, there is not yet an expert consensus to guide molecular pathological diagnostics of tumor using qPCR techniques in terms of validation and verification of method performance, quality management and interpretation of complex results. Therefore, this expert consensus aims to provide standardized opinions on the practical application of qPCR techniques, and suggestions on how to deal with common problems and abnormal results, and reach a Chinese expert consensus on the clinical practice of qPCR techniques in molecular pathological diagnostics of tumor, in order to standardize the testing process, improve the accuracy of results, and promote the clinical applications of qPCR techniques.