摘要目的 探讨皮肤滚针联合聚桂醇对毛细血管畸形的作用特点.方法 1.5 ～2.0 kg雄性莱航鸡24只,随机分为3组,每组8只.A组:皮肤滚针+聚桂醇;B组:皮肤滚针+生理盐水;C组:正常鸡冠.分别在皮肤滚针联合聚桂醇干预鸡冠后的第7、14、21、28天切取实验区域的鸡冠组织,制作病理切片后行HE染色、免疫组化染色和维多利亚蓝-丽春红染色,分别计算毛细血管数目和Ⅰ型、Ⅲ型胶原的纤维面积、弹力纤维面积.结果 肉眼观察:鸡冠组织的颜色随时间的推移而变浅;HE染色显示:A组毛细血管数目随时间的延长明显减少;Ⅰ型胶原纤维及Ⅲ型胶原纤维面积百分比计算结果:B组与A、C组对比,差异有统计学意义(P＜0.05);特殊染色结果显示:B组弹力纤维的表达有增生趋势.结论 动物实验显示,皮肤滚针联合聚桂醇干预鸡冠组织后可以有效地破坏、栓塞毛细血管网,且不导致局部组织的纤维化;单独应用皮肤滚针可以刺激真皮层弹力纤维轻度增生,可以延缓皮肤衰老的自然过程.

abstractsObjective To explore the effect of microneedle combined with Lauromacrogol on skin capillary network.Methods 24 male Leghone (1.5-2.0 kg in weight) were randomly divided into three groups as group A (microneedle combined with Lauromacrogol),B (microneedle combined with physiological saline),and C(control).The cockscombs were treated.The specimens were taken on the 7th,14th,21th,and 28th day postoperatively.HE staining,immunohistochemical staining and special staining were performed for study of the number of capillary and collagen Ⅰ /Ⅱ,as well as elastic fibers.Results The color of cockscombs in group A became lightening after treatment.The number of capillary decreased as showing by HE staining.The collagen Ⅰ and Ⅲ in group B was significantly different from that in group A and C (P ＜ 0.05).Special staining showed proliferation of elastic fibers in group B.Conclusions It indicates that microneedle combined with Lauromacrogol could effectively reduce the capillary in cockscomb without any tissue fibrosis.Microneedle can stimulate the proliferation of elastic fiber,so as to improve the skin ageing process.