摘要目的:探讨多配体蛋白聚糖-1(SDC1)在人静脉畸形组织中的表达，以及沉默SDC1表达对人脐静脉内皮细胞(HUVECs)迁移、侵袭及血管生成能力的影响。方法:静脉畸形病理标本取自2020年12月至2021年6月郑州大学第三附属医院血管瘤外科手术切除的20例病变组织(患者男10例，女10例，年龄1~57岁，中位年龄7岁)；HUVECs购自美国模式培养物集存库(ATCC)。采用免疫组化方法检测静脉畸形组织中SDC1的表达情况。体外培养HUVECs细胞学实验均分为2组进行，分别转染SDC1的小干扰RNA(siRNA)(si-SDC1组)和对照siRNA(对照组)。采用实时定量PCR法和蛋白质印迹法筛选、验证所合成siRNA对SDC1表达的沉默效果并用于后续实验。分别通过划痕实验、Transwell迁移和侵袭实验、血管生成实验检测HUVECs的迁移、侵袭、血管生成能力。结果:(1)20例静脉畸形组织中，13例(65.0%)SDC1呈阳性表达，且主要表达于静脉内皮细胞胞质。(2)与对照组HUVECs相比，si-SDC1组HUVECs的SDC1 mRNA和蛋白表达均降低( P<0.05)，说明所合成针对SDC1的siRNA可以沉默SDC1的表达。(3)与对照组相比，si-SDC1组HUVECs的迁移、侵袭和血管生成能力均增强( P<0.05)。 结论:SDC1在静脉畸形组织中多呈阳性表达，沉默SDC1表达后，HUVECs的迁移、侵袭和血管生成能力增强。

abstractsObjective:To investigate the expression of syndecan-1 (SDC1) in human venous malformations and the effect of SDC1 silencing on the migration, invasion and angiogenesis abilities of human umbilical vein endothelial cells (HUVECs).Methods:Twenty surgically resected samples of venous malformation were collected from 20 patients with hemangioma at the Third Affiliated Hospital of Zhengzhou University from December 2020 to June 2021 (10 males and 10 females, aged 1-57 years, median age 7 years). HUVECs were purchased from American Type Culture Collection (ATCC). Immunohistochemistry staining was used to detect the expression of SDC1 in venous malformations. HUVECs cultured in vitro were divided into two groups, and were transfected with SDC1 small interfering RNA (siRNA) (si-SDC1 group) or control siRNA (control group), respectively. The siRNA with the best silencing effect was screened and tested by real-time quantitative PCR and Western blotting for follow-up experiments. The migration, invasion and angiogenesis abilities of HUVECs were measured by scratch test, Transwell migration and invasion test, and angiogenesis test, respectively. Results:(1) SDC1 was positive in 13(65.0%) of the 20 venous malformations and was mainly expressed in the cytoplasm of venous endothelial cells. (2) Compared with HUVECs in the control group, the expression of SDC1 mRNA and protein in HUVECs cells in si-SDC1 group both decreased ( P<0.05), indicating the synthesized siRNA targeting SDC1 could silence the expression of SDC1. (3) The migration, invasion and angiogenesis abilities of HUVECs were enhanced in si-SDC1 group( P<0.05). Conclusions:SDC1 was expressed in most venous malformations. After silencing SDC1, the migration, invasion and tube formation abilities of HUVECs were enhanced.