摘要Heat stress transcription factors (Hsfs) are the central regulators of defense response to heat stress. We identified a total of 25 rice Hsfgenes by genome-wide analysis office (Oryza sativa L.) genome, including the subspecies of O.japonica and O. indica. Proteins encoded by OsHsfs were divided into three classes according to their structures. Digital Northern analysis showed that OsHsfs were expressed constitutively. The expressions of these OsHsfs in response to heat stress and oxidative stress differed among the members of the gene family. Promoter analysis identified a number of stress-related cis-elements in the promoter regions of these OsHsfs. No sign i ficant correlation, however, was found between the heat-shock responses of genes and their c/s-elements. Overall, our results provide a foundation for future research of OsHsfs function.