首页 > 浙江大学学报（英文版）（B辑：生物医学和生物技术） > Overexpression of the steroidogenic acute regulatory protein increases the expression of ATP-binding cassette transporters in microvascular endothelial cells(bEnd.3)

Overexpression of the steroidogenic acute regulatory protein increases the expression of ATP-binding cassette transporters in microvascular endothelial cells(bEnd.3)

导出在线阅读解读下载全文终端阅读

二维码有效期 120s

点击刷新

注：终端设备浏览有效期：

学术成果认领

翻译打印收藏纠错

摘要Objective: To determine the effect of steroidogenic acute regulatory protein(StAR)overexpression on the levels of adenosine triphosphate(ATP)-binding cassette transporter A1(ABCA1)and ATP-binding cassette transporter G1(ABCG1)in an endothelial cell line(bEnd.3).Methods: The StAR gene was induced in bEnd.3 cells with adenovirus infection.The infection efficiency was detected by fluorescence activated cell sorter(FACS)and fluorescence microscopy.The expressions of StAR gene and protein levels were detected by real-time polymerase chain reaction(PCR)and Western blot.The gene and protein levels of ABCA1 and ABCG1 were detected by real-time PCR and Western blot after StAR overexpression.Results: The result shows that StAR was successfully overexpressed in bEnd.3 cells by adenovirus infection.The mRNA and protein expressions of ABCA1 and ABCG1 were greatly increased by StAR overexpression in bEnd.3 cells.Conclusion: Overexpression of StAR increases ABCA1 and ABCG1expressions in endothelial cells.