摘要Objective:To explore the effects of insulin-like growth factor-1 (IGF-1) on migration,proliferation and differentiation of mesenchymal stem cells (MSCs).Methods:MSCs were obtained from Sprague-Dawley rats by a combination of gradient centrifugation and cell culture techniques and treated with IGF-1 at concentrations of 5-20 ng/ml.Proliferation of MSCs was determined as the mean doubling time.Expression of CXC chemokine receptor 4 (CXCR4)and migration property were determined by flow cytometry and transwell migration essay,respectively.mRNA expression of GATA-4 and collagen ∥ was determined by reverse transcription-polymerase chain reaction (RT-PCR).Results:The mean doubling time of MSC proliferation was decreased,and the expression of CXCR4 on MSCs and migration of MSCs were increased by IGF-1,all in a dose-dependent manner,while the optimal concentration of IGF-1 on proliferation and migration was different.IGF-1 did not affect the expression of GATA-4 or collagen ∥ mRNA.Conclusions:IGF-1 dose-dependently stimulated the proliferation of MSCs,upregulated the expression of CXCR4,and accelerated migration.There was no apparent differentiation of MSCs to cardiomyocytes or chondrocytes after culturing with IGF-1 alone.