纳米银颗粒粘附对牙科合金细胞毒性和抗菌性的影响

Effects on cytotoxicity and antibacterial properties of the incorporations of silver nanoparticles into the surface coating of dental alloys

摘要目的:评估纳米银颗粒的粘附对牙科合金的细胞毒性和抗菌性的影响,并初步探讨其作用机制.创新点:运用MTT法证实钴铬合金和纯钛对小鼠成骨前体细胞(MC3T3-E1)及大鼠骨髓间充质干细胞(BMSC)产生细胞毒性,粘附纳米银颗粒后细胞毒性有所降低.方法:将化学法制得的3种浓度的纳米银颗粒分别粘附于6种牙科合金表面,扫描电镜观察并确认纳米银的粘附情况.采用MTT法检测不同浓度纳米银颗粒的牙科合金对MC3T3-E1及BMSC的细胞毒性.评价3种浓度纳米银颗粒的钴铬合金和纯钛试件浸提液对金黄色葡萄球菌和变形链球菌的抗菌性.结论:牙科合金对MC3T3-E1和BMSC细胞具有较强的毒性,粘附纳米银颗粒后细胞毒性有所降低.3种浓度的纳米银颗粒细胞毒性之间无显著性差异,且这3种浓度纳米银颗粒粘附后对牙科合金的抗菌性无明显影响.

作者单位 Stomatology Hospital Affiliated to Zhejiang University of Medicine, Hangzhou 310006, China ^[1] Department of General Dentistry, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China ^[2] Department of Stomatology, Hangzhou First People's Hospital, Hangzhou 310006, China ^[3] College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China ^[4]

关键词纳米银颗粒牙科合金细胞毒性抗菌性能 MC3T3-E1细胞 BMSC细胞 Silver nanoparticles (AgNPs)Dental casting Cytotoxicity Antibacterial MC3T3-E1 BMSC

主题词细胞(Cells)银(Silver)牙科合金(Dental Alloys)性(Sex)铬合金(Chromium Alloys)

分类号 R783.1

栏目名称 Articles

发布时间 2017-07-25

基金项目

the Public Welfare Projects of Science Tech-nology Department of Zhejiang Province the Natural Science Foundation of Zhejiang Province China

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