首页 > 浙江大学学报（英文版）（B辑：生物医学和生物技术） > 软珊瑚 Sarcophyton glaucum中新型细胞毒性寡肽的纯化与鉴定

软珊瑚 Sarcophyton glaucum中新型细胞毒性寡肽的纯化与鉴定

Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum

摘要目的:纯化和鉴定软珊瑚Sarcophyton glaucum蛋白水解物中的细胞毒性肽.创新点:首次从软珊瑚S. glaucum中发现具有癌细胞毒性的寡肽,开发了一种有潜力的新型抗癌药物.方法:通过碱性蛋白酶、胰凝乳蛋白酶、木瓜蛋白酶和胰蛋白酶产生软珊瑚S. glaucum蛋白质水解物,并通过 MTT法评估其对人宫颈癌 HeLa细胞的毒性.通过膜超滤、凝胶过滤色谱、固相萃取和反相高效液相色谱等技术进一步提纯肽;采用从头测序法(de novo sequencing)进行肽鉴定;根据所鉴定的序列,进一步确定合成肽的细胞毒性.结论:从软珊瑚S. glaucum的木瓜蛋白酶水解物中鉴定出三种新型肽序列:AGAPGG、AERQ和RDTQ (分子量分别为428.45、502.53和518.53 Da).此三种寡肽具有对HeLa的细胞毒性,其半最大效应浓度(EC50)值各为8.6、4.9和5.6 mmol/L,为抗癌药物5-氟尿嘧啶的3.3、5.8和5.1倍.当测试对非癌细胞Hek293的毒性时,这三种肽仅显示16%、25%和11%的细胞毒性.因此,AERQ、AGAPGG和RDTQ有很大的潜力成为肽类抗癌药物.

作者单位 Department of Chemical Science, Faculty of Science, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia ^[1] Department of Biological Science, Faculty of Science, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia;Centre for Biodiversity Research, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia ^[2] Department of Geography, Faculty of Arts and Social Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia ^[3] Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia;Institute of Ocean and Earth Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia ^[4] Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Malaysia ^[5] Centre for Biodiversity Research, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia;Department of Biomedical Science, Faculty of Science, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia ^[6] Department of Chemical Science, Faculty of Science, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia;Centre for Biodiversity Research, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia ^[7]

关键词抗癌治疗活性肽细胞毒性 HeLa细胞 Sarcophyton glaucum 软珊瑚 Anticancer therapy Bioactive peptide Cytotoxicity HeLa cells Sarcophyton glaucum Soft coral

医学主题词细胞(Cells)癌(Carcinoma)药用制剂(Pharmaceutical Preparations)蛋白(Egg White)木瓜蛋白酶(Papain)

分类号 Q516（蛋白质）

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发布时间 2019-03-05（万方平台首次上网日期，不代表论文的发表时间）

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