题目:IGF-1R/β-catenin信号通路在2型糖尿病性骨质疏松中的作用

IGF-1R/β-catenin signaling axis is involved in type 2 diabetic osteoporosis

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摘要目的:探讨胰岛素样生长因子-1受体(IGF-1R)/β-联蛋白(β-catenin)信号通路是否在糖尿病性骨质疏松(DOP)病理机制中起作用.创新点:发现IGF-1R/β-catenin信号通路在DOP病理机制中起作用,可能是DOP潜在的治疗靶点.方法:收集DOP患者血清,使用酶联免疫吸附测定(ELISA)法检测IGF-1R水平.DOP大鼠在4周高脂饲料喂养后给予链脲佐菌素建模,对照组大鼠在普通饲料喂养4周后再给予链脲佐菌素溶媒(柠檬酸钠缓冲液).应用双能X线吸收法、生物力学测试和苏木精-伊红(HE)染色法分别评估椎体骨量、骨强度和骨组织形态.使用实时定量聚合酶链反应(qRT-PCR)和蛋白印迹法(western blotting)测定IGF-1R、糖原合成酶激酶-3β(GSK-3β)和 β-catenin表达及其蛋白磷酸化水平.结论:DOP患者血清IGF-1R较对照组高.DOP大鼠骨量、压缩强度明显减小,HE染色显示DOP椎体骨组织形态明显受损,IGF-1R信使RNA(mRNA)表达上调,IGF-1R、GSK-3β 和 β-catenin蛋白磷酸化增加.由此可见,IGF-1R/β-catenin信号通路在DOP的病理机制中起作用,该发现将有利于后期DOP治疗靶点的开发.

作者单位 The First Clinical School, Guangzhou University of Chinese Medicine, Guangzhou 510405, China ^[1] Department of Spinal Surgery, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China ^[2] School of Basic Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China ^[3] Department of Spinal Surgery, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China;Lingnan Medical Research Center of Guangzhou University of Chinese Medicine, Guangzhou 510405, China ^[4]

关键词糖尿病性骨质疏松胰岛素样生长因子-1受体信号通路发病机制 Diabetic osteoporosis Insulin-like growth factor-1 receptor (IGF-1R)Signaling axis Pathogenesis

主题词性(Sex)骨质疏松(Osteoporosis)生长调节素类(Somatomedins)糖尿病(Diabetes Mellitus)糖尿病, 2型(Diabetes Mellitus, Type 2)

分类号 R587.2

栏目名称 Articles

发布时间 2019-09-16

基金项目

the National Natural Science Foundation of China the Natural Science Founda-tion of Guangdong Province the Science and Technology Projects of Guangdong Province the Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme China

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准确一般字义偏差语意偏离译文作用小提交