摘要Dietary consumption of eicosapentaenoic acid(EPA)offers diverse health benefits,such as the regulation of blood triglycerides and the prevention of cardiovascular diseases.EPA is naturally synthesized by Schizochytrium sp.;however,its low production level limits its potential for industrial application.The goal of this study was to increase EPA productivity in Schizochytrium sp.by gas?liquid-phase plasma(GLPP)mutagenesis combined with a high-throughput screening method.First,a diverse array of mutants was generated through GLPP mutagenesis.Next,the mutants with elevated EPA productivity were identified through near-infrared spectroscopy(NIRS).Notably,the M7-25 mutant demonstrated the highest and most consistent EPA production.After the culture medium was optimized,the EPA titer increased from 0.45 to 1.70 g/L.Finally,a cofermentation strategy using ammonia and glucose feeding was employed,and the EPA titer reached 2.08 g/L in a 7-L fermenter.This study reports the highest EPA titer achieved in Schizochytrium sp.via mutagenesis to date,highlighting its great market potential for industrial production.