摘要目的 研究苏木水提物对人类卵巢癌细胞SKOV3的诱导凋亡机制.方法 采用免疫细胞化学法测定不同质量浓度苏木水提物作用后SKOV3细胞中Caspase-3、Caspase-9及Survivin的表达.结果 随着苏木水提物质量浓度的增加Caspase-3、Caspase-9阳性表达率增加,灰度值降低;Survivin 阳性表达率降低,灰度值升高.苏木水提物质量物浓度为0、25、50、100、200 mg/L时,Caspase-3的阳性表达率依次为5.29%、12.61%、24.60%、100.00%、100.00%;Caspase-9的阳性表达率依次为12.32%、29.17%、57.42%、100.00%、100.00%;Survivin的阳性表达率依次为81.62%、60.53%、36.94%、55.69%、36.92%.结论 苏木水提物通过增加Caspase-3、Caspase-9的表达及抑制Survivin的表达而诱导SKOV3细胞凋亡.

abstractsObjective To investigate the mechanism of oaesalpinia sappan aqueous extract's apoptosis-inducing on human ovarian cancer cell line SKOV3.Methods With different concentration of eaesalpinia sappan aqueous extract acting on cells,immunocytochemistry was used to detect the expression of Caspase-3,9 and survivin assay in ovarian cancer cell line SKOV3.Results The positive rate of Caspase-3,9 were increased with the increasing concentration of caesalpinia sappan aqueous extract,while the gray scale was decreased.The positive rates of surviving were decreased with the increasing concentration of caesalpinia sappan aqueous extract,while the gray scale were increased.At the concentration of 0,25 μg/ml,50μg,ml,100μs/ml,200μg/ml,the positive rates of Caspase-3 were 5.29%,12.61%,24.60%,100.00%,100.00%,respectively;the positive rates of Caspase-9 were 12.32%,29.17%,57.42%,100.00%,100.00%,respectively;the positive rates of survivin were 8 1.62%,60.53%,36.94%,55.69%,36.92%,respectively.Conclusion Caesalpinia sappan aqueous extract can obviously induce apoptosis of human ovarian cancer ceil line SKOV3 by increasing expression of Caspase-3,9 and decreasing expression of survivin.