摘要目的 探讨肝细胞生长因子受体c-met及异质性胞核核糖核蛋白HnRNP A2/B1在非小细胞肺癌(NSCLC)组织中的表达及其与病理临床的关系.方法 选取手术切除的46例NSCLC组织标本和30例正常肺组织标本,采用半定量RT-PCR法检测c-met、HnRNP A2/B1基因的转录表达情况,并结合临床资料进行分析.结果 在NSCLC组中,c-met及HnRNP A2/B1的mRNA阳性率(65.2%比26.7%,60.9%比20.0%)及相对表达量[(0.903±0.04)比(0.205±0.06),(0.162±0.04)比(0.096±0.02)]均明显高于对照组,两组间比较差异有统计学意义(P＜0.05).这两种标志物联合检测的阳性率高于单一标志物检测,差异有统计学意义(P＜0.01).同时,c-met、HnRNP A2/B1基因mRNA表达情况与临床分级和病理N分期相关(P＜0.05),而与患者年龄、性别及病理类型等不相关(P＞0.05).结论 c-met及HnRNP A2/B1在NSCLC中高表达,检测这两种标志物对临床分期有一定的指示作用,联合检测能显著提高NSCLC诊断的敏感度.两种基因的表达突变可能与NSCLC的发生、发展有关.

abstractsObjective To investigate the mRNA expression level of the hepatocyte growth factor receptor, c-met, and heterogeneous nuclear ribonucleoprotein, HnRNPA2/B1, in non-small cell lung cancer (NSCLC) patients and their relationships with invasion and metastasis of NSCLC. Methods The mRNA expressions of the c-met and HnRNP A2/B1 in postoperative samples of 46 patients with NSCLC and tissue samples of 30 patients with lung innocence disease as normal controls were detected by RT-PCR, and the efficacies of each marker and combining both of markers in the diagnosis of NSCLC were analyzed by Chi square test. Results The positive rates and relative expression quantity of c-met [65.2 % and (0.903±0.04)]and HnRNP A2/B1 [60.9 % and (0.162±0.04)] in NSCLC were significantly higher than those in control group [26.7 %, (0.205±0.06) and 20.0 %, (0.096±0.02), respectively] (P ＜0.05), and the positive rate of combining both was higher than that of single marker for NSCLC diagnosis(P ＜0.05) . The overexpression of two markers was also significantly correlated with the N stage and clinical stage, but not with age, gender and pathologic types. Conclusion The high expression of c-met and HnRNP A2/B1 in NSCLC may be of significance for the clinical stage. Combining test of two markers provides more sensitivity for NSCLC diagnosis. The expression change of two markers may involve in the carcinogenesis and development of NSCLC.