摘要目的 分析免疫组织化学(IHC)、色素原位杂交(CISH)、荧光原位杂交(FISH)方法在乳腺浸润性导管癌组织表皮生长因子受体(Her-2)基因检测中的选择应用.方法 分别采用IHC、CISH、FISH方法对100例乳腺浸润性导管癌组织的Her-2基因进行检测.结果 IHC(-)、(+)、(++)、(+++)者与CISH结果符合率分为100%(6/6)、7.1%(1/14)、32%(16/50)、80%(24/30);CISH和FISH符合率为100%(20/20).结论 IHC、CISH、FISH方法各有优劣.CISH是一准确、可靠且较易普及开展的Her-2基因检测方法.

abstractsObjective Through comparision of Her-2 gene in invasive breast carcinoma tissues assayed by IHC, CISH and FISH respectively, to explore an optimal detective methods. Methods IHC, CISH and FISH were used in detection for Her-2 gene of 100 cases, respectively.Results CISH identified no Her-2 gene amplification in IHC score negative rumors (6/6) and gene amplication in 7.1%(1/14) of IHC score (+) and in 32 % of IHC score (++) tumors(l6/50) and in 80 % of IHC score (+++) (24/80), Concordant rate between CISH and FISH was 100 %(20/20). Conclusion There are advantage and disadvantage among IHC,CISH and FISH. CISH is an accurate and dependable methods and would be easy to apply for detection of Her-2 gene.