摘要目的 探讨Dysadherin在肾透明细胞癌(RCCC)中的表达情况及其临床意义.方法 选取60例新鲜RCCC组织及其癌旁肾组织.患者男35例,女25例;年龄37～78岁,中位年龄61岁;肿块直径＞7 cm 24例,≤7 cm 36例;核分级Ⅰ、Ⅱ级39例,Ⅲ、Ⅳ级21例,应用反转录-聚合酶链反应( RT-PCR)及免疫组织化学的方法分别在mRNA及蛋白水平检测Dysadherin的表达情况.结果 RCCC组织中Dysadherin mRNA表达水平为2.0043±0.2890,明显高于癌旁肾组织的0.8461±0.2479(t=6.8020,P＜0.05).Dysadherin在RCCC中的表达与肿瘤的核分级有关,其中核分级为Ⅲ、Ⅳ级的表达量明显高于Ⅰ、Ⅱ级,二者mRNA表达量分别为1.6224±0.3194、2.7780±0.2288(t=6.5750,P＜0.05),蛋白表达阳性率分别为64.1％(25/39)和95.2 ％(20/21)( x2=5.495,P＜ 0.05),而在患者不同性别(t=1.0530,x2=0.023)、年龄(t=0.0511,x2=0.089)、肿块直径(t=1.0330,x2=0.370)之间差异无统计学意义(P＞0.05).结论 Dysadherin的表达随着RCCC肿瘤细胞核分级的升高而升高,可能成为RCCC有价值的预后标志物之一.

abstractsObjective To investigate the expression of Dysadherin and analyze its role in renal clear cell carcinoma (RCCC).Methods RT-PCR and immunohistochemical were used to detect the expression of Dysadherin in 60 cases of fresh RCCC and 60 adjacent normal renal tissues(male 35,female 25; age 37-78,median age 61; ＞7 cm 24,≤7 cm 36; Ⅰ/Ⅱ 39,Ⅲ/Ⅳ 21).Results Dysadherin mRNA expression in RCCC tissues (2.0043±0.2890) was higher than that in adjacent normal renal tissues (0.8461 ±0.2479) (t =6.8020,P ＜ 0.05).Dysadherin expression was associated with nuclear grade.The expression of Dysadherin in nucleus grade Ⅲ and Ⅳ tumors were significantly higher than that in nucleus grade Ⅰ and Ⅱ tumors [the mRNA expression were 4.6224±0.3194,2.7780±0.2288,the positive rates of protein were 64.1％ (25/39),95.2 ％ (20/21) (t =6.5750,x2 =5.495,P ＜ 0.05)].There was no association between the expression of Dysadherin with sex (t =1.0530,x2 =0.023),age(t =0.0511,x2 =0.089) and tumor size (t =1.0330,x2 =0.370) (P ＞ 0.05).Conclusion In RCCC,Dysadherin expression is positively associated with tumor aggressiveness based on grading.It seems that Dysadherin may be a valuable prognostic marker in RCCC.