摘要目的 观察巴西木素对人类膀胱癌T24细胞增殖和凋亡的影响.方法 采用锥虫蓝拒染法观察不同浓度巴西木素在不同时间对靶细胞的增殖抑制作用；采用均质生物发光法检测靶细胞内Caspase-3、9的活性；用流式细胞术及激光共聚焦显微镜检测巴西木素对靶细胞的凋亡作用情况.结果 不同浓度巴西木素(25、50、100、200 μg/ml)与靶细胞作用8h,细胞抑制率分别为43.19％、60.73％、86.38％、93.89％(P＜ 0.05),50 μg/ml的巴西木素与靶细胞作用4、8、24、48 h后,细胞抑制率分别为52.72％、60.73％、91.77％、96.41％(P＜ 0.05)；生物发光检测25μg/ml的巴西木素作用后靶细胞Caspase-3、9的活性略有升高,但与对照组比较差异无统计学意义(P＞0.05)；巴西木素与靶细胞作用16h后,在7.5 ～ 60.0 μg/ml范围内随着药物浓度增加细胞凋亡率分别为0.15％、1.35％、2.91％、34.76％,呈现上升趋势；激光共聚焦显微镜下可见靶细胞发生凋亡.结论 巴西木素对人类膀胱癌细胞T24具有显著的抑制作用,并诱导其发生凋亡,随药物作用呈现一定的浓度时间依赖性.

abstractsObjective To observe the effects of brazilin on proliferation and apoptosis in T24 cells.Methods Trypan blue exclusion test was performed to detect the inhibition of brazilin on the growth of T24 cell lines in vitro cultured within different time.After exposure to different concentrations of brazilin,homogeneous bioluminescence assay was used to detect the inhibitory action of brazilin,Caspase-3 and Caspase-9 activity on T24 cells.Cellular apoptosis was measured by flow cytometry (FCM) and observed by laser scanning confocal microscope.Results Brazilin could significantly inhibit the proliferation of T24 cells after 8 hours,the inhibitory rates of the brazilin at concentration of 25,50,100,200 μg/ml against T24 cells respectively were 43.19 ％,60.73 ％,86.38 ％ and 93.89 ％ (P ＜ 0.05).After exposured to 50 μg/ml of brazilin,the inhibition ration to T24 cells increased with time prolonging (52.72 ％ in 4 h,60.73 ％ in 8 h,91.77 ％ in 24 h,96.41％ in 48 h) (P ＜ 0.05).The activity of Caspase-3 and Caspase-9 increased slightly when brazilin was at 25 μg/ml,but there was no statistical differences compared with that in the control group (P ＞ 0.05).When cells were treated with an increase of the concentration of brazilin from range of 7.5-60 μg/ml for 16 hours,the apoptosis ratio in turn showed a upward trend of 0.15 ％,1.35 ％,2.91％,34.76 ％.It could be seen by laser scanning confocal microscope that the apoptosis occurred in the cells.Conclusion Brazilin can effectively inhibit the proliferation of T24 cells and induce apoptosis in a dose and time dependent manner.