摘要目的 探讨MAGE-A9在人类肝细胞癌中的表达及其与肝细胞癌患者临床病理特征之间的关系.方法 应用反转录聚合酶链反应(RT-PCR)、实时荧光定量核酸扩增检测(qPCR)和免疫组织化学方法分析MAGE-A9在肝癌细胞株和组织标本中的表达.使用Kaplan-Meier统计方法和Cox比例风险回归模型来进行生存分析,评估100例肝细胞癌患者的预后.结果 MAGE-A9 mRNA在肝细胞癌中的表达(4.44±0.34)明显高于非肿瘤细胞和组织(1.73±0.18) (P＜ 0.05),MAGE-A9的表达与病理分级(P=0.003)、门静脉侵犯(P=0.001)、远处转移(P=0.022)和TNM分期(P=0.005)有关.Cox回归分析进一步表明,MAGE-A9表达是无病生存期(P=0.006)和总生存期(P=0.022)的独立预后因素.结论 MAGE-A9是与肝细胞癌患者预后相关的有价值的生物标志物,MAGE-A9高表达的肝细胞癌患者预后较差.

abstractsObjective To detect the expression of MAGE-A9 in human hepatocellular carcinoma (HCC), and to investigate the association between expression of MAGE-A9 and the clinicopathological characteristics of HCC.Methods Reverse transcription-polymerase chain reaction (RT-PCR), one-step quantitative-PCR (qPCR) and immunohistochemistry (IHC) methods were performed to characterize the expression of MAGE-A9 in HCC cell lines and tissues.Kaplan-Meier survival analysis and Cox regression were employed to evaluate the prognosis of 100 HCC patients.Results The expression of MAGE-A9 mRNA in HCC (4.44±0.342) was significantly higher than that in non-cancerous cells and tissues (1.73±0.178) (P ＜ 0.05).The expression level of the MAGE-A9 protein in HCC was related to the pathological grade (P =0.003), portal vein invasion (P =0.001), distant metastasis (P =0.022) and TNM stage (P =0.005).Cox regression analysis revealed that MAGE-A9 expression is an independent prognostic factor for disease-free survival (P =0.006) and overall survival (P =0.022).Conclusion MAGE-A9 is a valuable prognostic biomarker for HCC patients, and its high expression suggests unfavorable survival outcomes in HCC patients.