肺部隐球菌病检测的病理染色方法改进
Pathologic staining method improvement for detection of pulmonary cryptococcal disease
摘要目的 探讨标记肺隐球菌病的病理学染色方法.方法 分别采用传统六胺银染色和酸化高锰酸钾溶液六胺银染色分析已确诊肺部隐球菌感染的51例患者的石蜡切片.结果 应用酸化高锰酸钾溶液六胺银染色均阳性,全过程只需25min,背景清晰,肺隐球菌包囊结构清晰可辨.结论 酸化高锰酸钾溶液六胺银染色比传统六胺银染色操作简便易行、效果良好,值得推广应用.
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abstractsObjective To investigate the pathological staining method of pulmonary cryptococcal disease. Methods Traditional hexamine silver staining and acidified potassium permanganate solution hexamine silver stained were used to analyze paraffin sections of 51 patients with pulmonary cryptococcal disease. Results Compared with traditional hexamine silver stained, the result of acidified potassium permanganate solution with hexamine silver stained was positive, and the whole process only required 25 minutes with the clear background and cryptococcal cyst capsule structure. Conclusion Compared with the traditional hexamine silver staining, acidification of potassium permanganate solution hexamine silver staining method is simpler,easier,more effective and worthy of wide application.
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