摘要目的:探讨连接斑珠蛋白（JUP）体外对放疗抵抗子宫颈癌细胞的影响。方法:对子宫颈癌细胞株SiHa、HeLa、Me-180采用单次剂量1 Gy、每周3次的 60Co放射线照射，诱导细胞耐放疗性，得到获得性放疗抵抗细胞株SiHaIR、HeLaIR、Me-180IR，以相应野生型细胞株作为对照组。分别采用实时荧光定量聚合酶链反应（RT-qPCR）、蛋白质印迹法检测6组细胞中JUP mRNA及蛋白的表达情况，采用细胞划痕实验检测细胞迁移能力。 结果:SiHa组和SiHaIR组、HeLa组和HeLaIR组、Me-180组和Me-180IR组JUP mRNA相对表达量分别为1.74±0.06和0.48±0.02（ t=12.327， P<0.01）、1.77±0.06和0.28±0.03（ t=14.698， P<0.01）、2.28±0.06和0.78±0.01（ t=7.367， P<0.01），JUP蛋白的相对表达量分别为2.36±0.03和0.55±0.02（ t=9.245， P<0.01）、2.13±0.02和0.23±0.01（ t=15.643， P<0.01）、1.96±0.05和0.73±0.02（ t=5.826， P<0.01）。划痕后培养12、24、48 h时，3组放疗抵抗细胞迁移率均较相应野生型细胞增加，差异均有统计学意义（均 P<0.05）。 结论:JUP在放疗抵抗子宫颈癌细胞株的表达量低于野生型细胞株，且放疗抵抗细胞的迁移能力增强。

abstractsObjective:To explore the effect of junctional plakoglobin (JUP) on the radiotherapy-resistant cervical cancer cells in vitro.Methods:Cervical cancer cell lines SiHa, HeLa and Me-180 were irradiated with 1 Gy of 60Co radioactive rays for 3 times every week to induce the radiotherapy resistance of cells which obtained the radiotherapy-resistant cell lines SiHaIR, HeLaIR and Me-180IR. The corresponding wild-type cell lines were served as control groups. Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and Western blot were used to detect the expressions of JUP mRNA and protein in the 6 groups of cells. The cell scratch test was used to detect the cell migration ability. Results:The relative expressions of JUP mRNA in SiHa and SiHaIR groups, HeLa and HeLaIR groups and Me-180 and Me-180IR groups were the relative expressions of JUP protein were 1.74±0.06 and 0.48±0.02 ( t = 12.327, P < 0.01), 1.77±0.06 and 0.28±0.03 ( t = 14.698, P < 0.01), 2.276±0.061 and 0.780±0.011 ( t = 7.367, P < 0.01); 2.36±0.03 and 0.55±0.02 ( t = 9.245, P < 0.01), 2.13±0.02 and 0.23±0.01 ( t = 15.643, P < 0.01), 1.96±0.05 and 0.73±0.02 ( t = 5.826, P < 0.01). When culturing the cells for 12, 24, and 48 h after scratching, the migration rates in 3 groups of radiotherapy-resistant cell lines were increased compared with the corresponding wild-type cell lines, and the differences were statistically significant (all P < 0.05). Conclusion:The expressions of JUP in radiotherapy-resistant cervical cancer cell lines are lower than those in wild-type cell lines, and the migration ability of radiotherapy-resistant cells is enhanced.