摘要Background:The aim of this study was to investigate the effects of different minimally invasive surgical procedures on intestinal muco-sal barrier function.Methods:In this study,76 patients who underwent minimally invasive gastric cancer surgery were selected,and peripheral blood was collected to test the levels of serum plasma D-lactic acid,diamine oxidase,and bacterial endotoxin before and 1 and 3 days after sur-gery.These markers were compared at different time points before and after surgery to understand the recovery of the intestinal muco-sal barrier function in patients after surgery.Results:On the first postoperative day,the change in serum D-lactic acid relative to the preoperative levels was significantly(P＜0.05)lower in the laparoscopic surgery group(4.05[-0.195,6.917 mmoVL])than in the robot-assisted surgery group(7.56[5.190,12.145 mmol/L]).Both the serum D-lactic acid and bacterial endotoxin levels were significantly higher on the first postoperative day compared with preoperative levels,and although they showed a gradual decrease by the third day,they remained significantly higher than the pre-operative levels(P＜0.05).The Student-Newman-Keuls method for pairwise comparison of the measurements at each time point dem-onstrated that the differences in D-lactic acid and bacterial endotoxin levels between the preoperative sample and the sample collected on the third postoperative day were statistically significant(P＜0.05).Conclusions:Compared with the laparoscopic surgery group,the robotic surgery group showed larger changes in the postoperative serum D-lactic acid level,suggesting that the robotic surgery resulted in greater damage to the barrier function of the intestinal mucosa.The serum D-lactic acid and bacterial endotoxin levels were significantly higher in postoperative patients and showed a trend to gradually decrease,suggesting that the intestinal mucosal barrier function of patients after minimally invasive gastric cancer surgery is damaged and then gradually recovers.