摘要Accurate prediction of phenotypes across genotypes and environments is crucial for accelerating crop improvement.Process-based crop growth models(CGMs)can capture complex genotype-by-environment in-teractions,but their use is limited by labor-intensive genotypic parameter measurements.Here,we developed a faster data assimilation pipeline integrating high-throughput phenotyping(HTP)observations with the Sir-iusQuality wheat model to efficiently estimate key genotypic parameters and predict genotype performance.Using time-series RGB imagery from a ground-based Phenomobile,we assimilated intercepted photosynthetically active radiation(fIPAR),heading date,and final grain yield to jointly assimilated to calibrate twelve genotypic parameters governing phenology,canopy development,light interception,biomass accumulation,and grain filling.Two data assimilation strategies—a Bayesian DREAM(zs)algorithm and a lookup table(LUT)inver-sion—were compared through both in silico experiment and eight years of multi-environment field trials of nine durum wheat cultivars.The LUT method demonstrated superior computational efficiency,with prediction ac-curacy comparable to Bayesian inference on real field data.Multi-year field trials showed that two environments(year/site)were sufficient to reliably characterize genotypic parameters and predict performance across envi-ronments.By combining time-series HTP data with ecophysiological modeling,our data assimilation pipeline offers breeders a powerful tool for genotype characterization.It streamlines the process of capturing environ-mental variance and phenotypic stability,reducing time and effort in crop improvement.