DNA Polymorphism Among Yewei B, V20B, and Oryza minuta J. S. Presl. ex C. B. Presl.

二维码有效期 120s

摘要The new cytoplasmic male sterile (CMS) line YeweiA and its maintainer line Yewei B, with better agronomic characteristics, have been developed from a mutant of V20B (a rice maintainer line) through transformation of genomic DNA of wild rice (Oryza minuta J. S. Presl. ex C. B. Presl.). Analysis of molecular markers, DNA sequences, and Southern blot revealed that high DNA polymorphism exists between the mutant and its receptor, indicating that the special DNA fragment from O. minuta may be integrated into the genome of Yewei B. Therefore, transformation of genomic DNA from distant relatives to the plant of a target receptor may open an avenue for creating a new rice germplasm.

作者单位 China National Hybrid Rice R & D Center, Changsha 410125, China ^[1] Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing 100010, China ^[2] Tianjin Agriculture College, Tianjin 300384, China ^[3]

关键词 DNA sequence genomic DNA introduction germplasm molecular markers wild rice

分类号 Q94

发布时间 2006-03-02

基金项目

Grants from the Hunan Science and Technology Commission HunanAcademy of Agriculture Science and Technology

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