A Novel Approach to Functional Analysis of the Ribulose Bisphosphate Carboxylase Small Subunit Gene by Agrobacterium-Mediated Gene Silencing

二维码有效期 120s

摘要A novel approach to virus-induced post-transcriptional gene silencing for studying the function of the ribulose bisphosphate carboxylase small subunlt (rbcS) gene was established and optimized using potato virus X vector and Nicotiana benthamiana as experimental material. The analysis of silencing phenomena,transcriptional level, protein expression, and pigment measurement showed that the expression of the rbcS endogenous gene was inactivated by the expression of a 500-bp homologous cDNA fragment carried in the virus vector.

作者单位 Institute of Genetics and Cytology,Northeast Normal University,Changchun 130024,China;Institute of Gene Technology,Jilin Normal University,Siping 136000,China ^[1] Institute of Genetics and Cytology,Northeast Normal University,Changchun 130024,China ^[2]

关键词 Agrobacterium-mediated transient expression potato virus X ribulose bisphosphate carboxylase small subunit (rbcS) gene virus-induced gene silencing

分类号 Q94

栏目名称

Molecular and Developmental Biology

发布时间 2006-11-07

基金项目

国家自然科学基金（IRT0519）； the Chinese National Program for Changjiang Scholars and Innovative Research Team (PCSIRT) in University； Plant Transformation Center； Jilin Provincial Science and Technology Commission

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