High-throughput Binary Vectors for Plant Gene Function Analysis

二维码有效期 120s

摘要A series of high-throughput binary cloning vectors were constructed to facilitate gene function analysis in higher plants. This vector series consists of plasmids designed for plant expression, promoter analysis, gene silencing,and green fluorescent protein fusions for protein localization. These vectors provide for high-throughput and efficient cloning utilizing sites for λ phage integrase/excisionase. In addition, unique restriction sites are incorporated in a multiple cloning site and enable promoter replacement. The entire vector series are available with complete sequence information and detailed annotations and are freely distributed to the scientific community for non-commercial uses.

作者单位 School of Life Sciences, University of Science and Technology of China, Hefei 230027, China ^[1] State Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China ^[2] Department of Genetics, Development and Cell Biology, lowa State University, Ames, IA 50011, USA ^[3]

关键词 binary vectors gene silencing green fluorescent protein fusion high-throughput promoter analysis recombinationassisted cloning

分类号 Q94

栏目名称 NEW TECHNOLOGY

发布时间 2007-04-29

基金项目

the Rockefeller Foundation Food Security Program 国家高技术研究发展计划(863计划) 中国科学院基金 United States Department of Agriculture National Research Initiative Competitive Program

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