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Xanthophyll Cycle and Inactivation of Photosystem Ⅱ Reaction Centers Alleviating Reducing Pressure to Photosystem Ⅰ in Morning Glory Leaves under Short-term High Irradiance

摘要investigated through chlorophyll fluorescence parameters in morning glory (Ipomoea setosa) leaves, which were dipped into water, dithiothreitol (DTT) and lincomycin (LM), respectively. During the stress, both the xanthophyll cycle and D1 protein turnover could protect PSI from photoinhibition. In DTT leaves, non-photochemical quenching (NPQ) was inhibited greatly and the oxidation level of P700 (P700+) was the lowest one. However, the maximal photochemical efficiency of PSII (Fv/Fm) in DTT leaves was higher than that of LM leaves and was lower than that of control leaves. These results suggested that PSI was more sensitive to the loss of the xanthophyll cycle than PSII under high irradiance. In LM leaves, NPQ was partly inhibited, Fv/Fm was the lowest one among three treatments under high irradiance and P700+ was at a similar level as that of control leaves. These results implied that inactivation of PSII reaction centers could protect PSI from further photoinhibition. Additionally, the lowest of the number of active reaction centers to one inactive reaction center for a PSII cross-section (RC/CSo), maximal trapping rate in a PSII cross-section (TRo/CSo), electron transport in a PSII cross-section (ETo/CSo) and the highest of 1-qP in LM leaves further indicated that severe photoinhibition of PSII in LM leaves was mainly induced by inactivation of PSII reaction centers, which limited electrons transporting to PSI. However, relative to the LM leaves the higher level of RC/CSo, TRo/CSo, Fv/Fm and the lower level of 1-qP in DTT leaves indicated that PSI photoinhibition was mainly induced by the electron accumulation at the PSI acceptor side, which induced the decrease of P700+ under high irradiance.

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作者单位 High-Tech Research Center, Shandong Academy of Agricultural Sciences, Ji'nan 250100, China [1] Ji'nan Entry-exit Inspection Quarantine Bureau of the People's Republic of China, Ji'nan 250100, China [2] High-Tech Research Center, Shandong Academy of Agricultural Sciences, Ji'nan 250100, China;Vegatable Institute, Shangdong Academy of Agricultural Sciences, Ji'nan 250100,China [3]
分类号 Q94
栏目名称 Development & Photosynthesis
发布时间 2007-09-24
基金项目
国家自然科学基金 the Scientific Research Encouragement Foundation for Outstanding Young and Middle Scientists of Shandong Province
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植物学报(英文版)

植物学报(英文版)

2007年49卷7期

1047-1053页

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