HP30-2, a mitochondrial PRAT protein for import of signal sequence-less precursor proteins in Arabidopsis thaliana

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摘要Chloroplasts and mitochondria contain a family of putative preprotein and amino acid transporters designated PRAT.Here,we analyzed the role of two previously characterized PRAT protein family members,encoded by At3g49560(HP30) and At5g24650 (HP30-2),in planta using a combination of genetic,cell biological and biochemical approaches.Expression studies and green fluorescent protein tagging identified HP30-2 both in chloroplasts and mitochondria,whereas HP30 was located exclusively in chloroplasts.Biochemical evidence was obtained for an association of mitochondrial HP30-2 with two distinct protein complexes,one containing the inner membrane translocase TIM22 and the other containing an alternative NAD(P)H dehydrogenase subunit (NDC1)implicated in a respiratory complex 1-like electron transport chain.Through its association with TIM22,HP30-2 is involved in the uptake of carrier proteins and other,hydrophobic membrane proteins lacking cleavable N H2-terminal presequences,whereas H P30-2's interaction with NDC1 may permit controlling mitochondrial biogenesis and activity.

作者单位 Laboratory of Plant Molecular Genetics and Laboratory of Environmental and Systems Biology, Grenoble-Alpes-University, Grenoble, France ^[1] Department of Biological Sciences, University of Toledo, 2801 West Bancroft Street, Toledo, OH 43606, USA ^[2] Department of Plant and Environmental Sciences, Pee Dee Research and Education Center, Clemson University, Florence, SC 29506, USA;Department of Crop and Soil Sciences, Washington State University, Pullman WA 99164-6420, USA ^[3] Department of Crop and Soil Sciences, Washington State University, Pullman WA 99164-6420, USA ^[4]

栏目名称

Cell and Developmental Biology

DOI 10.1111/jipb.12555

发布时间 2017-10-10

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