Development of an efficient plant dual cytosine and adenine editor

二维码有效期 120s

摘要An enhanced CDA-like (eCDAL) was established from Japanese lamprey CDA1-like 4 to achieve a high editing frequency in a broad region as a C-terminal cytosine base editors (CT-CBE). Then, a novel plant dual-base editor version 1(pDuBE1) was developed by integrating TadA-8e into eCDAL. The editing efficiency of pDuBE1 could reach to 87.6％, with frequencies of concurrent A-to-G and C-to-T conversions as high as 49.7％ in stably transformed plant cells. Our results showed that pDuBE1 could mediate robust dual editing in plant genome, providing a powerful manipulation tool for precise crop breeding and screening platforms for in planta direct evolution.

作者 Rongfang Xu ^[1] Fanna Kong ^[1] Ruiying Qin ^[1] Juan Li ^[1] Xiaoshuang Liu ^[2] Pengcheng Wei ^[2] 学术成果认领

作者单位 Key Laboratory of Rice Genetic Breeding of Anhui Province,Rice Research Institute,Anhui Academy of Agricultural Sciences,Hefei 230031,China ^[1] Key Laboratory of Rice Genetic Breeding of Anhui Province,Rice Research Institute,Anhui Academy of Agricultural Sciences,Hefei 230031,China;College of Agronomy,Anhui Agricultural University,Hefei 230036,China ^[2]

关键词 base editing CRISPR dual-base editor genome editing rice

栏目名称 Breakthrough Reports

发布时间 2021-09-28

基金项目

This work was funded by the National Natural Science Foundation of China Natural Science Foundation of Anhui province Open Research Fund Program of Anhui Province Key Laboratory of Rice Genetics and Breeding Science and Technology Major Projects of Anhui Province and Key Technology Research Project of Hefei

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