摘要Anthocyanins are important natural plant pigments and play diverse roles in plant growth and adapta-tion.Anthocyanins function as screens to protect photosynthetic tissues from photoinhibition.How-ever,the regulatory mechanisms underlying the bi-osynthesis and spatial accumulation pattern of anthocyanins remain some unresolved issues.Here,we demonstrate that the GARP-type transcription factor GOLDEN2-LIKE 1(GLK1)functions as a pos-itive factor in anthocyanin accumulation.GLK1 en-hances the transcriptional activation activities of MYB75,MYB90,and MYB113 via direct protein-protein interactions to increase the expression of anthocyanin-specific biosynthetic genes.Anthocya-nins accumulate in an acropetal manner in Arabi-dopsis.We also found that the expression pattern of GLK1 overall mimicked the accumulation pattern of anthocyanin from the base of the main stem to the shoot apex.Based on these findings,we estab-lished a working model for the role of GLK1 in an-thocyanin accumulation and propose that GLK1 mediates the spatial distribution pattern of antho-cyanins by affecting the transcriptional activation activities of MYB75,MYB90,and MYB113.