摘要Fusarium crown rot(FCR)and sharp eyespot(SE)are serious soil-borne diseases in wheat and its relatives that have been reported to cause wheat yield losses in many areas.In this study,the ex-pression of a cell wall invertase gene,TaCWI-B1,was identified to be associated with FCR resist-ance through a combination of bulk segregant RNA sequencing and genome resequencing in a recombinant inbred line population.Two bi-parental populations were developed to further verify TaCWI-B1 association with FCR resistance.Overexpression lines and ethyl methanesulfonate(EMS)mutants revealed TaCWI-B1 positively regulating FCR resistance.Determination of cell wall thickness and components showed that the TaCWI-B1-overexpression lines exhibited considerably increased thickness and pectin and cellulose contents.Furthermore,we found that TaCWI-B1 directly interacted with an alpha-galactosidase(TaGAL).EMS mutants showed that TaGAL negatively modulated FCR resistance.The expression of TaGAL is negatively correlated with TaCWI-B1 levels,thus may reduce mannan deg-radation in the cell wall,consequently leading to thickening of the cell wall.Additionally,TaCWI-B1-overexpression lines and TaGAL mutants showed higher resistance to SE;however,TaCWI-B1 mu-tants were more susceptible to SE than controls.This study provides insights into a FCR and SE resistance gene to combat soil-borne diseases in common wheat.