摘要Phosphorus is a major nutrient vital for plant growth and development,with a substantial amount of cellular phosphorus being used for the biosynthesis of membrane phospholipids.Here,we report that NON-SPECIFIC PHOSPHOLIPASE C4(NPC4)in rapeseed(Brassica napus)releases phosphate from phospholipids to promote growth and seed yield,as plants with altered NPC4 levels showed significant changes in seed production under different phosphate conditions.Clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated nuclease 9(Cas9)-mediated knockout of BnaNPC4 led to elevated accumulation of phospholipids and decreased growth,whereas overexpression(OE)of BnaNPC4 resulted in lower phospholipid contents and increased plant growth and seed production.We demonstrate that BnaNPC4 hydrolyzes phos-phosphingolipids and phosphoglycerolipids in vitro,and plants with altered BnaNPC4 function displayed changes in their sphingolipid and glyc-erolipid contents in roots,with a greater change in glycerolipids than sphingolipids in leaves,partic-ularly under phosphate deficiency conditions.In addition,BnaNPC4-OE plants led to the upregu-lation of genes involved in lipid metabolism,phosphate release,and phosphate transport and an increase in free inorganic phosphate in leaves.These results indicate that BnaNPC4 hydrolyzes phosphosphingolipids and phosphoglycerolipids in rapeseed to enhance phosphate release from membrane phospholipids and promote growth and seed production.