摘要Maize(Zea mays)requires substantial amounts of nitrogen,posing a challenge for its cultivation.Re-cent work discovered that some ancient Mexican maize landraces harbored diazotrophic bacteria in mucilage secreted by their aerial roots.To see if this trait is retained in modern maize,we con-ducted a field study of aerial root mucilage(ARM)in 258 inbred lines.We observed that ARM secretion is common in modern maize,but the amount sig-nificantly varies,and only a few lines have retained the nitrogen-fixing traits found in ancient landraces.The mucilage of the high-ARM inbred line HN5-724 had high nitrogen-fixing enzyme activity and abundant diazotrophic bacteria.Our genome-wide association study identified 17 candidate genes associated with ARM across three environments.Knockouts of one candidate gene,the subtilase family gene ZmSBT3,confirmed that it negatively regulates ARM secretion.Notably,the ZmSBT3 knockout lines had increased biomass and total nitrogen accumulation under nitrogen-free culture conditions.High ARM was associated with three ZmSBT3 haplotypes that were gradually lost during maize domestication,being retained in only a few modern inbred lines such as HN5-724.In summary,our results identify ZmSBT3 as a potential tool for enhancing ARM,and thus nitrogen fixation,in maize.