摘要Objective:It has been reported that local vibration therapy can benefit recovery after peripheral nerve injury,but the optimized parameters and effective mechanism were unclear.In the present study,we investigated the effect of local vibration therapy of different amplitudes on the recovery of nerve function in rats with sciatic nerve injury(SNI).Methods:Adult male Sprague-Dawley rats were subjected to SNI and then randomly divided into 5 groups:sham group,SNI group,SNI+A-1 mm group,SNI+A-2 mm group,and SNI+A-4 mm group(A refers to the amplitude;n=10 per group).Starting on the 7th day after model initiation,local vibration therapy was given for 21 consecutive days with a frequency of 10 Hz and an amplitude of 1,2 or 4 mm for 5 min.The sciatic function index(SFI)was assessed before surgery and on the 7th,14th,21st and 28th days after surgery.Tissues were harvested on the 28th day after surgery for morphological,immunoflu-orescence and Western blot analysis.Results:Compared with the SNI group,on the 28th day after surgery,the SFIs of the treatment groups were increased;the difference in the SNI+A-2 mm group was the most obvious(95％confidence interval[CI]:[5.86,27.09],P＜0.001),and the cross-sectional areas of myocytes in all of the treatment groups were improved.The G-ratios in the SNI+A-1 mm group and SNI+A-2 mm group were reduced signif-icantly(95％CI:[-0.12,-0.02],P=0.007;95％CI:[-0.15,-0.06],P＜0.001).In addition,the expressions of S100 and nerve growth factor proteins in the treatment groups were increased;the phosphorylation expressions of ERK1/2 protein in the SNI+A-2 mm group and SNI+A-4 mm group were upregulated(95％CI:[0.03,0.96],P=0.038;95％CI:[0.01,0.94],P=0.047,respectively),and the phosphorylation expression of Akt in the SNI+A-1 mm group was upregulated(95％CI:[0.11,2.07],P=0.031).Conclusion:Local vibration therapy,especially with medium amplitude,was able to promote the recov-ery of nerve function in rats with SNI;this result was linked to the proliferation of Schwann cells and the activation of the ERK1/2 and Akt signaling pathways.